~1H-NMR代谢组学技术用于牛黄解毒片中对雄黄具有配伍减毒作用的主要化学成分群的研究  

~1H-NMR-based metabonomics on chemical component groups with toxicity alleviation effect to Realgar in Niuhuang Jiedu tablet

作　　者：徐文峰[1] 裴月湖[2] 

机构地区：[1]北京医院药学部,北京100730 [2]沈阳药科大学基于靶点的药物设计与研究教育部重点实验室,辽宁沈阳110016

出　　处：《中国中药杂志》2016年第12期2228-2234,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81073035)

摘　　要：利用~1H-NMR为基础的代谢组学技术,探讨牛黄解毒片中对雄黄具有配伍减毒作用的主要化学成分群。将24只大鼠随机分成4组:空白组、R组(雄黄组)、RRSPG组(雄黄配伍大黄、黄芩、桔梗和甘草组)、RC组(雄黄配伍大黄总蒽醌、黄芩总黄酮、桔梗总皂苷、甘草总黄酮和甘草总皂苷组),对各组大鼠尿液及血清样品~1H-NMR谱图进行模式识别分析。结果显示雄黄组大鼠尿液及血清样品代谢轮廓和空白组差异明显,RC组大鼠代谢轮廓同空白组相似,向空白组回归,RC组各化学成分可通过调节能量代谢、胆碱代谢、氨基酸代谢和肠道微生物代谢等方面而起到降低雄黄毒性的作用。To study the chemical component groups with toxicity alleviation effect to Realgar in Niuhuang Jiedu tablet based on^1H-NMR metabonomics. Twenty-four male Wistar rats were divided into four groups： control group,R group（ treated with Realgar）,RRSPG group（ treated with Realgar,the root and rhizoma of Rheum palmatum,the root of Scutellaria baicalensis,the root of Platycodon grandiflorum and the root and rhizoma of Glycyrrhiza uralensis） and RC group（ treated with total anthraquinones from the root and rhizoma of R. palmatum,total flavonoids from the root of S. baicalensis,total saponins from the root of P.grandiflorum,total flavonoids and saponins from the root and rhizoma of G. uralensis）. Based on^1H-NMR spectra of urine and serum from rats,PLS-DA was performed to identify different metabolic profiles. The metabolic profiles of R group were different from that of control group,while the metabolic profiles of RC group were almost similar to control group. Total anthraquinones from the root and rhizoma of R. palmatum,total flavonoids from the root of S. baicalensis,total saponins from the root of P. grandiflorum,total flavonoids and saponins from the root and rhizoma of G. uralensis regulated energy,choline and amino acid metabolism and gut flora disorder affected by realgar＇ s toxicity.

关 键 词：代谢组学 牛黄解毒片 雄黄 化学成分群 配伍减毒 

分 类 号：R285.5[医药卫生—中药学]