野生冬虫夏草水提物体外抗HIV-1病毒作用的初步研究  

In Vitro Anti-HIV-1 Activity of Cordyceps sinensis Extracts

作　　者：朱莹[1] 马莉[1] 胡秦[1] 李劲涛[1,2] 陈玉龙[3] 贾润清[1] 沈思嗣[1] 曾毅[1,2] 

机构地区：[1]北京工业大学生命科学与生物工程学院环境与病毒肿瘤学北京市重点实验室 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室 [3]四川省林业科学研究院

出　　处：《病毒学报》2016年第4期417-422,共6页Chinese Journal of Virology

基　　金：北京市自然科学基金(7142013);国家自然科学基金(81202976);传染病预防控制国家重点实验室自主研究重点课题(2011SKLID103);"艾滋病和病毒性肝炎等重大传染病防治"国家科技重大专项;艾滋病治疗型疫苗研制(2012ZX10001005)

摘　　要：研究分析野生冬虫夏草的水溶浸提物抑制人免疫缺陷病毒Ⅰ型(human immunodeficiency virus-1,HIV-1)的效果及其抗病毒作用的靶点。研究分别选取新鲜虫体、子座、全草、干燥虫体和子座5种不同组分,获得水溶性提取物,采用CCK-8实验检测水提物的细胞毒性;假病毒单周期感染实验评价水提物的体外抗病毒活性;体外检测水提物对逆转录酶活性的影响,应用表面等离子共振(SPR)技术检测水提物与HIV-1Vif蛋白的相互作用。这5种虫草水提物均具有显著的体外抗HIV-1病毒活性,抑制HIV-1逆转录酶的活性,新鲜子座水提物与Vif蛋白有良好的体外结合力。本实验明确了野生冬虫夏草的体外抗病毒活性,其作用机制可能与抑制逆转录酶活性和体外Vif蛋白有关,研究将为抗HIV-1病毒药物的研发提供实验基础。To investigate the in vitro anti-HIV-1effect of the aqueous extracts of Cordyceps sinensis.The aqueous stroma and sclerotium extracts were isolated from the fresh and dry Cordyceps sinensis specimen,respectively.The CCK-8test and the TZM-bl pseudovirus assay were used to examine the in-vitro cytotoxicity and anti-HIV-1activities of extracts.In addition,the reverse-transcriptase enzyme-activity assay and the surface plasma resonance（SPR）technology were taken to study the inhibition on the activity of reverse transcriptase and interaction with Vif protein.All 5aqueous extracts of Cordyceps sinensis exhibited in vitro anti-HIV-1effects,extracts from the fresh fungus showed more potent effect in inhibiting reversetranscriptase activity than the dry fungus.Furthermore,a strong interaction was observed between the fresh stroma extract and Vif protein.The study clarified that the in vitro anti-HIV-1activity of the aqueous extracts of Cordyceps sinensis,may be mediated through inhibition of reverse-transcriptase activity and interaction with Vif protein.

关 键 词：冬虫夏草 HIV-1病毒 逆转录酶 Vif蛋白 

分 类 号：R373[医药卫生—病原生物学]