2013～2014年中国大陆四株不同基因型麻疹病毒分离株血凝素基因特征分析  

Genetic Characteristic Analysis of H Gene among Four Genotypes of MeasIes Viruses Isolated in China's Mainland during 2013-2014

作　　者：任丽[1] 王慧玲[1] 王常银[2] 房学强[2] 许文波[1] 王英[1] 张燕[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]山东省疾病预防控制中心病毒病预防控制所,济南250014

出　　处：《病毒学报》2016年第4期453-458,共6页Chinese Journal of Virology

基　　金：国家自然科学基金课题(项目号:81371791);国家科学技术重大专项艾滋病和病毒性肝炎等重大传染病防治课题(编号:2012ZX10004215;2013ZX10004202;2012ZX10004401;2012ZX10004201-003);世界卫生组织西太平洋区麻疹参比实验室的职能(WP CHN AAA 011XZ 08)

摘　　要：研究2013～2014年中国大陆分离到4株不同基因型麻疹野毒株血凝素蛋白（HA）编码基因的基因和氨基酸特征及变异程度。选取2013～2014年我国优势基因型H1a1株,输入基因型B3、D8和D9各1株作为代表株,核酸提取,采用逆转录-聚合酶链反应（RT-PCR）扩增H基因编码区全序列（1 854bp）并测序,使用sequencher5.0进行序列拼接,并与GenBank下载的H1基因型4株、B3基因型和D8基因型各8株、D9基因型2株及中国A基因型麻疹疫苗株沪191（S191）和长47（C47）H基因全序列进行比较分析,使用MEGA5.0比对并构建亲缘性关系树及分析其核苷酸和氨基酸差异。4株不同基因型野毒株与A基因型疫苗株H基因核苷酸和氨基酸同源性为94.2%～96.7%,95.4%～96.7%,氨基酸差异数为20～28,其中Beijing14-1（H1a）与A基因型疫苗株H基因同源性最低。H1a毒株与1993～1994年分离的毒株核苷酸同源性较高为97.7%～98.2%。Beijing14-1（H1a）和Shanghai14-1（B3）第240位点氨基酸位点由S→N,导致HA蛋白的1个N-糖基化位点丢失。4株野毒株与中国A基因型疫苗株H基因核苷酸和氨基酸同源性较高,且重要中和抗原位点未出现明显突变。从基因层面上分析A基因型疫苗株对我国流行的H1a亚型及B3、D8和D9输入性基因型野毒株感染仍具有较好的保护效果。To study the genetic characterization and amino acid mutation of hemagglutinin protein of four genotypes of wild type measles Viruses isolated in 2013 and 2014year,in China,including H1,B3,D8 and D9.Four genotype isolates of H1,D8,D9 and B3measles viruses were selected,and RNA of MV isolates were extracted.The complete sequence of hemagglutinin（1854nt）were amplified by RT-PCR and sequenced and Sequencher5.0was used to splice sequence.Phylogenetic analysis and the diversity of gene and amino acid were done by MEGA version 5.0,compared with 24 representative strains,repectively 4 H1 a sub-genotype strains,8D8 genotype strains,2D9 genotype strains,8B3 genotype strains and 2Chinese vaccine Strains,which were downloaded from GenBank.The homology of H gene nucleotide sequence and amino acid between the four genotype measles virus strains including H1,B3,D8,D9 and A genotype strains were respectively 94.2%-96.7%and 95.4%-96.7%and there were 20-28 amino acids difference between them;And the homology of H gene nucleotide sequence between Beijing14-1（H1a）and A genotype vaccine strains was 97.7%-98.2%.The N-glycosylation site in 240 th was losen because of mutation.The homology of H gene nucleotide sequence and amino acid between the four genotype measles virus strains including H1,B3,D8,D9 and A genotype strains were high,and the Chinese vaccine can effectively prevent infection caused by H1asub-genotype,D8,D9 and B3imported genotypes virus strains.

关 键 词：血凝素蛋白 麻疹病毒 基因特征 输入性 

分 类 号：R373.9[医药卫生—病原生物学]