胶原诱导性关节炎小鼠脾脏及踝关节中G蛋白耦联受体激酶2的表达  

Expression of G protein-coupled receptor kinase 2 in spleen and ankle joints from collagen-induced arthritis mice

作　　者：王婷婷[1] 刘燕[1] 王小琴[1] 邱一华[1] 

机构地区：[1]南通大学医学院生理学系,南通226001

出　　处：《南通大学学报（医学版）》2016年第1期1-4,F0002,共5页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(31371182)

摘　　要：目的 :了解胶原诱导性关节炎(collagen-induced arthritis,CIA)发病后免疫器官及免疫细胞内G蛋白耦联受体激酶2(G protein-coupled receptor kinase 2,GRK2)的表达及其变化,探讨其参与CIA发病的机制。方法:用鸡Ⅱ型胶原(collagen typeⅡ,CⅡ)免疫DBA/1J小鼠;免疫荧光双标法检测小鼠踝关节和脾脏中CD4、Foxp3、ROR-γt分别和GRK2的共定位;Western Blot法检测正常小鼠和CIA小鼠踝关节和脾脏中GRK2的表达变化。结果 :CIA小鼠四肢踝关节明显红肿;正常小鼠与CIA小鼠脾脏组织中均有CD4、Foxp3、ROR-γt和GRK2的共定位;CIA小鼠踝关节中也有CD4、Foxp3、ROR-γt和GRK2的共定位;CIA小鼠脾脏和踝关节组织中的GRK2的表达均降低。结论 :CIA小鼠脾脏及踝关节组织表达GRK2且GRK2的表达显著低于正常小鼠。Objective： To investigate the expressions and changes of G protein-coupled receptor kinase 2（GRK2） in immune organs and immune cells from collagen-induced arthritis（CIA） mice, and to explore the pathogenesis of GRK2 involved in the of CIA. Methods： CIA was induced by chicken type Ⅱ collagen（C Ⅱ ） in DBA/1J; Colocalization of CD4, Foxp3, or ROR-γt, and GRK2 from ankle and spleen in normal and CIA mice were detected by double immunofluorescent staining respectively; GRK2 expression in ankle and spleen from normal and CIA mice were determined by Western Blot. Results： Significant swelling of ankle joint was observed in CIA models, colocalization of CD4, Foxp3, ROR-γt and GRK2 in spleen from both normal and CIA mice were observed respectively; colocalization of CD4, Foxp3, ROR-γt and GRK2 were also detected in ankle tissue from CIA mice models; during the inflammatory process, the expression of GRK2 was downregulated from the tissue of spleen and ankle joint of CIA. Conclusions： GRK2 was expressed in spleen and ankle joint tissues from CIA and normal mice, the level of GRK2 expression was profoundly decreased in CIA compared with normal mice.

关 键 词：胶原诱导性关节炎 G蛋白耦联受体激酶2 CD4细胞 调节性T细胞 辅助性T细胞17 小鼠 

分 类 号：R684.3[医药卫生—骨科学]