紫外-可见分光光度法测定茶枯多糖的含量  

Determination of Polysaccharide in Chaku by Ultraviolet and Visible Spectrophotometry

作　　者：刘潇潇[1] 张丽丹[1] 李华[1] 陈浩桉[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《今日药学》2016年第6期384-385,396,共3页Pharmacy Today

基　　金：广东省省级科技计划项目(2013B040200029)

摘　　要：目的建立茶枯中糖类成分的含量测定方法。方法采用紫外-可见分光光度法,样品提取后用苯酚-硫酸法显色,于486 nm波长处测定吸光度,通过随行标准曲线法计算含量。结果本法线性关系良好（r=0.999 7）,回收率在96.1%~102.5%之间,RSD为2.3%。结论该法简便快速,可用于茶枯中糖类成分的测定,为茶枯质量标准的建立提供思路。OBJECTIVE To establish a method for the determination of polysaccharide in Chaku. METHODS Ultraviolet and visible spectrophotometry was selected to determine the content of polysaccharide in Chaku. The extracted samples were determined by Phenol-H2SO4 colorimetry method and measured the absorbance at 486 nm. The content of Chaku polysaccharide was counted with the accompanying standard curve. RESULTS The calibration curves showed good linear regression（ r = 0. 999 7）. The recoveries were between 96.1% and 102.5%,with RSD 2.3%. CONCLUSION The method was simple and fast,suitable for the content determination of polysaccharide in Chaku. It provides helpful information for quality control of Chaku.

关 键 词：茶枯多糖 苯酚-硫酸法 含量测定 

分 类 号：R284[医药卫生—中药学]