家鸡卵泡刺激素受体新型剪接变体(FSHR-v)的克隆及功能探究  

Cloning and Functional Analysis of a Novel Splice Variant of Follicle-Stimulating Hormone Receptor(FSHR)in Chickens

作　　者：何晨[1] 高顺玉[1] 黄龙[1] 孟风艳[1] 卜贵鲜[1] 王亚军[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2016年第4期902-908,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31271325;31272436)

摘　　要：以家鸡为材料,利用RT-PCR技术,从家鸡卵巢中克隆了卵泡刺激素受体的一种新型剪接变体(cFSHR-v).基因结构与氨基酸序列比对分析表明,该剪接变体在8号外显子的5′端发生了差别剪切,导致了其相比于正常形式FSHR多出21个核苷酸,由此推测该变体(cFSHR-v)可以编码含有700个氨基酸残基的受体蛋白.与正常形式cFSHR相比较,cFSHR-v在N端胞外域增加了7个氨基酸残基.采用RT-PCR技术和pGL3-CRE荧光素酶报告系统,本研究也探究了cFSHR-v的功能及其在家鸡组织中的表达情况.结果显示,FSHR在家鸡卵巢及精巢组织中均有较高表达水平,但在CHO细胞中表达的cFSHR-v不能有效介导卵泡刺激素(FSH)对下游cAMP-PKA信号通路的刺激效应,说明在家鸡性腺中,FSHR存在剪切变体形式.In this study, a novel splice variant of FSHR （cFSHR-v） was identified from chicken ovary by RT-PCR. cFSHR-v is predicted to encode a putative protein of 700 amino acids with an alternated extra- cellular domain. Comparison of this splice variant with chicken genome database revealed that it is resul- ted from partial sequence （21bp） retention of intron 7. RT-PCR assay showed that cFSHR-v could be detected in ovary and testis. Using a pGL3-CRE-Luciferase reporting system, we demonstrated that only cFSHR, but not cFSHR-v, expressed in cultured CHO cells could be activated by human FSH in a dose-dependent manner, indicating that cFSHR-v may be incapable of transmitting signal. The data revealed that FSHR variant is expressed in chicken gonads.

关 键 词：家鸡 卵泡刺激素受体 剪接变体 性腺 功能探究 

分 类 号：Q452[生物学—生理学]