UPLC-MS法测定中度嗜盐菌中的四氢嘧啶与羟基四氢嘧啶的方法优化  

The condition optimization determination of ectoine and hydroxyectoine in halophilic bacteria by UPLC-MS detection technology

作　　者：李海宇[1] 彭彤[1] 白林含[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2016年第4期919-924,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技支撑项目(2011GZ0001;2013JZ 0009)

摘　　要：本文通过对样品制备、提取方法和检测参数的研究,建立并优化了四氢嘧啶与羟基四氢嘧啶的超高效液相色谱（UPLC）的检测方法：使用UPLC HSS T3色谱柱,选择流动相为乙腈：水=1∶99,进样体积为1μL,流速为0.4mL/min,检测波长为204nm,柱温为40℃.四氢嘧啶及羟基四氢嘧啶在0.00391-5mg/L范围内可呈现良好线性关系（R^2=0.9997）,且回收率高,准确率强。An efficient UPLC method for determination ectoine and hydroxyectoine was develo;ped by studying the sample preparation, sample extraction and detection parameters. In the UPLC method, acetonitrile ： water （1 ： 99 v/v） as a mobile phase which through at a constant flow rate of 0.4 mL/min was performed on UPLC HSS T3 column, the detection wavelength was 204nm, the injection volume was 1μL, the temperature of the column was 40℃, the standard curve shows a good linear relation（R^2 =0. 9997）, and the results have a high recoveries of ectoine and hydroectoine. UPLC method is a higher sensitivity, shorter detection time, lower demand for sample volume and higher accuracy than HPLC and NMR technology.

关 键 词：中度嗜盐菌 四氢嘧啶 羟基四氢嘧啶 超高效液相色谱(UPLC) 

分 类 号：Q93-338[生物学—微生物学]