猪整合素β3多克隆抗体制备与鉴定  

Preparation and identification of polyclonal antibody against porcine integrin β3

作　　者：高晶[1] 王志慧[1] 郭东华[1] 李春秋[1] 苏明俊 王欣宇[1] 赵喜文[1] 王恩雨[1] 魏姗[1] 刘秋瑾[1] 孙东波[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2016年第7期25-28,275,276,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金面上项目(31472209)

摘　　要：为了制备猪整合素β3多克隆抗体,试验利用大肠杆菌原核表达系统,对密码子优化的猪整合素β3基因主要抗原区进行原核表达,表达的重组蛋白纯化后,免疫Balb/c雌性小鼠,采血后分离血清,进一步通过ELISA和Western-blot对制备的猪整合素β3多克隆抗体进行鉴定。结果表明:猪整合素β3基因主要抗原区获得了成功表达,表达蛋白分子质量为102 ku左右,以纯化重组蛋白作为免疫原制备的多克隆抗体效价为1∶12 800,且能识别天然猪整合素β3。To prepare the polyclonal antibody against porcine integrin β3, the prokaryotic expression of codon - optimized major antigen region of porcine integrin β3 was conducted using prokaryotic expression system of Escherichia coli. The Balb/c mice were immunized using the purified recombinant porcine integrin β3 protein, and then blood samples were collected to isolate serum. The ELISA and Western - blot were used for identification of the prepared polyclonal antibody against porcine integriu β3. The results showed that the major antigen region of porcine integrin β3 gene was successfully expressed, and the molecular weight of expressed protein was about 102 ku. The titer of the polyclonal antibody against porcine integjin β3 using the prepared recombinant protein as an immunogen was 1：12 800. Furthermore, the prepared polyclonal anti- body could recognize native porcine integrin β3.

关 键 词：猪 整合素Β3 原核表达 重组蛋白 多克隆抗体 病毒受体 病毒感染 

分 类 号：Q344.13[生物学—遗传学]