不同营养水平对大足黑山羊血浆中FSH与LH水平的影响  

Effects of different nutrition levels on FSH and LH in plasma of Dazu black goats

作　　者：刘灵丽[1] 陈璐[1] 唐天强[1] 赵中权[1] 

机构地区：[1]西南大学动物科技学院重庆市牧草与草食家畜重点实验室/重庆市草食动物资源保护与利用工程技术研究中心,重庆400716

出　　处：《黑龙江畜牧兽医》2016年第7期114-116,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家级大学生创新创业训练计划项目(201410635010);重庆市农业科技成果转化项目(cstc2015jcsf-nycgzh B0012)

摘　　要：为了研究营养对大足黑山羊繁殖性能的影响,试验选择10只大足黑山羊进行不同营养水平饲喂试验,在发情间期每天采集2次血样,发情期每3 h采集1次血样,分离血浆后采用酶联免疫(ELISA)双抗体夹心法测定血浆中的促黄体素(LH)和促卵泡素(FSH)浓度。结果表明:在发情周期内,FSH和LH呈波动式分泌,发情前出现LH峰,发情期FSH出现最高峰值,高营养水平组大足黑山羊血浆中FSH和LH水平显著高于低营养组(P<0.05)。说明增加饲料营养水平能够提高大足黑山羊繁殖性能。To study the effect of nutrition on reproductive performance of Dazu black goats, 10 Dazu Black female goats were selected for feeding trials with different nutrition levels. The blood ＇samples were collected twice a day and once every 3h during dioestrus and oestrus, respectively. Plasma was obtained after centrifugation, and then FSH and LH concentrations in plasma were determined using double - antibody sandwich ELISA. The results showed that FSH and LI-I exhibited a pulsatile secretion during the estrous cycle, and there was a peak of LH concentration before the oestrus, and there was a highest peak of FSH concentration during oestrus. The FSH and LH concentrations in plasma of Dazu balck goats in the high nutrition -level group were significantly higher （P 〈0.05 ） than those in the low nutrition -level group. The results indicate that the reproductive performance of Dazu black goats can be improved by increasing feed nutrition levels.

关 键 词：大足黑山羊 营养水平 酶联免疫(ELISA) 促卵泡素(FSH) 促黄体素(LH) 发情期 繁殖性能 

分 类 号：S854.43[农业科学—临床兽医学] S827[农业科学—兽医学]