强痛宁对大鼠中枢脑区Na^+-K^+-ATP酶活性的影响  

Effect of Qiangtongning on Na^+-K^+-ATPase activity in central brain regions of rats

作　　者：尹柏双[1,2] 沙万里[1] 李宇航[3] 呼显生[1] 付连军[1] 

机构地区：[1]吉林农业科技学院动物医学学院,吉林吉林132101 [2]预防兽医学吉林省重点实验室,吉林吉林132101 [3]吉林农业大学动物科技学院,长春130118

出　　处：《黑龙江畜牧兽医》2016年第7期194-196,200,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31302150);吉林省科技厅发展计划项目(20140204062NY);吉林省教育厅"十二五"科学技术项目(吉教科合字2014379);吉林农业科技学院博士启动基金项目(吉农院合字2012307);吉林农业科技学院种子基金项目(吉农院合字2013905)

摘　　要：为了探讨强痛宁麻醉镇痛作用与中枢脑区Na^+-K^+-ATP酶的相关性,试验选取24只纯种SD大鼠随机分成对照组、诱导组、麻醉组和催醒组,于各时间点冰上采集大鼠各脑区,采用比色法测定Na^+-K^+-ATP酶活性。结果表明:药物作用后诱导期及麻醉期大鼠大脑皮质、小脑内Na^+-K^+-ATP酶活性显著低于对照组(P<0.01),海马、丘脑及脑干区Na^+-K^+-ATP酶活性与对照组比较极显著降低(P<0.01),催醒期各脑区Na^+-K^+-ATP酶活性恢复到麻醉前水平。说明强痛宁可引起大鼠各脑区Na^+-K^+-ATP酶活性降低,阻碍神经细胞对痛觉刺激信息的传导,产生了麻醉镇痛作用。To explore the correlation between the narcotic analgesic effects of Qiangtongning and Na ^＋ - K^ ＋ - ATPase in central brain regions, 24 pure - bred SD rats were randomly divided into a control group, an induction group, an anesthesia group and a wake group. Brain samples were collected from the rats on the ice at different time points. The activity of Na ^＋ - K ^＋ - ATPase in the samples was determined by using colorimetric assay. The results showed that the activity of Na^ ＋ - K^＋ - ATPase in cerebral cortex and cerebellum was extremely significantly lower （ P 〈 0.01 ） than that of the control group during the induction and anesthesia after administration ; the activity of Na^＋ - K ^＋ - ATPase in hippocampus, thalamus and brain stem was extremely significantly decreased （P 〈0.01 ） compared with that of the control group; the activity / of Na ^＋ - K^ ＋ - ATPasc in different brain regions were recovered to the level before anesthesia in the waking period. The results indicate that Qiangtongning can cause the activity of Na ^＋ - K ^＋ - ATPase in different brain regions to reduce in rats and hinders the nerve cells to transmit pain stimulus information, resulting in a narcotic analgesic effect.

关 键 词：强痛宁 麻醉 大鼠 中枢系统 脑区 NA^+-K^+-ATP酶 

分 类 号：S857.124[农业科学—临床兽医学] S858.91[农业科学—兽医学]