高糖环境下维生素D对人前脂肪细胞增殖分化的影响  

Influence of vitamin D on proliferation and differentiation of human preadipocytes in high-glucose con-dition

作　　者：张红霞[1] 原杰[2] 

机构地区：[1]山西医科大学附属人民医院内分泌科,太原030012 [2]山西医科大学附属人民医院CT室,太原030012

出　　处：《中华临床营养杂志》2016年第3期155-159,共5页Chinese Journal of Clinical Nutrition

基　　金：山西省科技攻关项目（20130313020-3）

摘　　要：目的：探讨在高糖环境下1，25（OH）2 D3（VD3）对人前脂肪细胞增殖分化的影响。方法原代培养人前脂肪细胞并诱导分化，分为对照组、高糖组、对照＋VD3组和高糖＋VD3组。采用油红O染色和异丙醇萃取法半定量检测细胞内脂肪含量；实时PCR法检测各组细胞维生素D受体（ VDR）、CCAAT／增强子结合蛋白a （C／EBPa）和过氧化物酶体增殖物激活受体γ（PPARγ） mRNA的表达水平。结果与对照组比较，高糖组、高糖＋VD3组细胞内脂肪含量明显增高（0.231±0.033比0.362±0.045， P＝0.037；0.231±0.033比0.358±0.057， P＝0.033）， VD3可显著减少对照组细胞脂肪含量（0.231±0.033比0.174±0.029， P＝0.016），但对高糖组脂肪含量无影响（0.362±0.045比0.358±0.057， P＝0.450）。与对照组比较，高糖组VDR mRNA表达水平明显降低（0.397±0.029比0.069±0.003， P＝0.022）， VD3可显著提高对照＋VD3组和高糖＋VD3组细胞 VDR mRNA 的表达水平（0.397±0.029比1.103±0.044， P＝0.035；0.069±0.003比0.152±0.021， P＝0.041）。与对照组比较，高糖组C／EBPa、 PPARγmRNA 的表达水平显著升高（0.135±0.048比0.788±0.063， P＝0.018；0.498±0.026比0.795±0.037， P＝0.021）， VD3可明显减少对照＋VD3组PPARγmRNA水平（0.498±0.026比0.237±0.011， P＝0.029），但对高糖＋VD3组C／EBPa mRNA （0.788±0.063比0.843±0.049， P＞0.05）、 PPARγmRNA （0.795±0.037比0.813±0.041， P＞0.05）以及对照＋VD3组的C／EBPa mRNA无影响（0.135±0.048比0.156±0.022， P均＞0.05）。结论在体外高糖环境下， VD3生物活性降低，不能发挥对人前脂肪细胞增殖分化的抑制作用。Objective To investigate the influence of 1,25（OH）2D3 （VD3） on proliferation and dif-ferentiation of human preadipocytes in high-glucose condition .Methods Primary culture of human preadipo-cytes were divided into 4 groups：the control group, high glucose group, control＋VD3 group, and high glucose＋VD3 group.Oil red O staining and extraction with isopropyl alcohol were employed to detect the total amount of fat in different groups .Real-time polymerase chain reaction was used to detect the mRNA levels of vitamin D receptor （VDR）, CCAAT/enhancer binding protein a （C/EBPa） and peroxisome proliferator activated receptor gamma （ PPARγ） in each group .Results Compared with the control group , the fat amount was significantly increased in the high glucose group （0.231 ±0.033 vs.0.362 ±0.045 , P=0.037 ） and high glucose ＋VD3 group （0.231 ±0.033 vs.0.358 ±0.057, P=0.033）, decreased in the control ＋VD3 group （0.231 ± 0.033 vs.0.174 ±0.029, P=0.016）, but no significant difference in the glucose＋VD3 group （0.362 ±0.045 vs.0.358 ±0.057, P=0.450）;VDR mRNA level was decreased in the high glucose group （0.397 ± 0.029 vs.0.069 ±0.003 , P=0.022 ） , but VD3 increased the VDR mRNA levels in both the control ＋VD3 group and the high glucose ＋VD3 group （0.397 ±0.029 vs.1.103 ±0.044 , P=0.035;0.069 ±0.003 vs. 0.152 ±0.021 , P=0.041 ）;the mRNA levels of C/EBPa and PPARγwere up-regulated in the high glucose group （0.135 ±0.048 vs.0.788 ±0.063, P=0.018;0.498 ±0.026 vs.0.795 ±0.037, P=0.021）, VD3 decreased the PPARγmRNA level in the control ＋VD3 group （ 0.498 ±0.026 vs.0.237 ±0.011 , P =0.029）, but had no significant effect on C/EBPa mRNA （0.788 ±0.063 vs.0.843 ±0.049, P〉0.05） and PPARγmRNA （0.795 ±0.037 vs.0.813 ±0.041, P〉0.05） expressions in the high glucose ＋VD3 group or C/EBPa mRNA in the control＋VD3 group （0.135 ±0.048 vs.0.156 ±0.022, P〉0.05）.Conclusion VD3 may lose the ability to inhibit the proliferation and diffe

关 键 词：维生素D 维生素D受体 肥胖 糖尿病 

分 类 号：R589.2[医药卫生—内分泌] R587.1[医药卫生—内科学]