Isoquercitrin suppresses the expression of histamine and pro-inflammatory cytokines by inhibiting the activation of MAP Kinases and NF-κB in human KU812 cells  

作　　者：LI Li ZHANG Xiao-Hui LIU Guang-Rong LIU Chang DONG Yin-Mao 

机构地区：[1]Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing100048,China [2]Infinitus(China)Company Ltd.,Guangzhou 510665,China

出　　处：《Chinese Journal of Natural Medicines》2016年第6期407-412,共6页中国天然药物（英文版）

基　　金：Foundation of Beijing Superiority People(D classification,2014000020124G034)

摘　　要：Mast cells and basophils are multifunctional effector cells that contain abundant secretory granules in their cytoplasm. Both cell types are involved in a variety of inflammatory and immune events, producing an array of inflammatory mediators, such as cytokines. The aim of the study was to examine whether isoquercitrin modulates allergic and inflammatory reactions in the human basophilic KU812 cells and to elucidate its influence on the phosphorylation of mitogen-activated protein kinase(MAPK) and nuclear factor(NF)-κB activation. The KU812 cells were stimulated with phorbol-12-myristate 13-acetate plus the calcium ionophore A23187(PMACI). The inhibitory effects of isoquercitrin on the productions of histamine and pro-inflammatory cytokines in the stimulated KU812 cells were measured using cytokine-specific enzyme-linked immunosorbent(ELISA) assays. Western blotting analysis was used to assess the effects of isoquercitrin on the MAPKs and NF-κB protein levels. Our results indicated that the isoquercitrin treatment of PMACI-stimulated KU812 cells significantly reduced the production of histamine and the pro-inflammatory cytokines, such as interleukin(IL)-6, IL-8, IL-1β, and tumor necrosis factor(TNF)-α. The treated cells exhibited decreased phosphorylation of extracellular signal-regulated kinase(ERK), revealing the role of ERK MAPK in isoquercitrin-mediated allergy inhibition. Furthermore, isoquercitrin suppressed the PMACI-mediated activation of NF-κB in the human basophil cells. In conclusion, the results from the present study provide insights into the potential therapeutic use of isoquercitrin for the treatment of inflammatory and allergic reactions.Mast cells and basophils are multifunctional effector cells that contain abundant secretory granules in their cytoplasm. Both cell types are involved in a variety of inflammatory and immune events, producing an array of inflammatory mediators, such as cytokines. The aim of the study was to examine whether isoquercitrin modulates allergic and inflammatory reactions in the human basophilic KU812 cells and to elucidate its influence on the phosphorylation of mitogen-activated protein kinase（MAPK） and nuclear factor（NF）-κB activation. The KU812 cells were stimulated with phorbol-12-myristate 13-acetate plus the calcium ionophore A23187（PMACI）. The inhibitory effects of isoquercitrin on the productions of histamine and pro-inflammatory cytokines in the stimulated KU812 cells were measured using cytokine-specific enzyme-linked immunosorbent（ELISA） assays. Western blotting analysis was used to assess the effects of isoquercitrin on the MAPKs and NF-κB protein levels. Our results indicated that the isoquercitrin treatment of PMACI-stimulated KU812 cells significantly reduced the production of histamine and the pro-inflammatory cytokines, such as interleukin（IL）-6, IL-8, IL-1β, and tumor necrosis factor（TNF）-α. The treated cells exhibited decreased phosphorylation of extracellular signal-regulated kinase（ERK）, revealing the role of ERK MAPK in isoquercitrin-mediated allergy inhibition. Furthermore, isoquercitrin suppressed the PMACI-mediated activation of NF-κB in the human basophil cells. In conclusion, the results from the present study provide insights into the potential therapeutic use of isoquercitrin for the treatment of inflammatory and allergic reactions.

关 键 词：Isoquercitrin KU812 cells HISTAMINE CYTOKINES Mitogen-activated protein kinase Nuclear factor-κB 

分 类 号：R965[医药卫生—药理学]