LC-MS法测定麦冬皂苷D’浓度的不确定度评定  

Uncertainty Evaluation on Determination of Ophiopogonin D'in Plasma by LC- MS

作　　者：董晓茜[1] 杨瑞[1] 张世良[1] 唐思[1] 夏素霞[1] 李国信[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《中华中医药学刊》2016年第7期1606-1608,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家"重大新药创制"科技重大专项项目(2012zx09303-017);国家临床重点专科建设项目(2013);国家中医药管理局中药学重点学科项目(2009);辽宁中医药大学杏林学者青蓝工程项目(2013)

摘　　要：目的:评定LC-MS法测定血浆中麦冬皂苷D’浓度的不确定度。方法:对测定方法的全过程进行分析,建立评定其不确定度的数学模型,确定影响不确定度的因素并对各个不确定度因素进行评估,计算合成不确定度并进行扩展。结果:置信概率P为95%时,血浆中低(35.10μg/L)、中(280.80μg/L)、高(702.00μg/L)浓度麦冬皂苷D’的扩展不确定度分别为:6.22、7.64、76.50μg/L。结论:本方法适用于LC-MS法测定人血浆中麦冬皂苷D’浓度的不确定度评定。Objective： To evaluate the uncertainty of ophiopogonin D＇in determination in human plasma by LC- MS.Methods： The uncertainty caused by various factors in the whole process of determination was analyzed. Mathematical model was established for the evaluation of uncertainty to identify influential factors of uncertainty and evaluate the uncertainty factors. The combined uncertainty was calculated and the expanded uncertainty was obtained. Results： The expanded uncertainty for the low（ 35. 10 μg / L）,medium（ 280. 80 μg / L） and high（ 702. 00 μg / L） level of ophiopogonin D＇was6. 22,7. 64 and 76. 50 μg / L,respectively（ P = 95%）. Conclusion： This method is applicable to uncertainty evaluation of ophiopogonin D＇determination in human plasma and the uncertainty of this method was mainly caused by linear regression.

关 键 词：生脉注射液 麦冬皂苷D’ LC-MS法 不确定度 

分 类 号：R284.1[医药卫生—中药学]