16S rDNA克隆文库分析含硫污水生物反应器中生物群落  

Analysis of biological communities in bioreactor of treating sulfide-containing wastewater by 16S rDNA cloning method

作　　者：张跃洋[1,2] 梁存珍[2] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100022 [2]北京石油化工学院环境工程系,北京102617

出　　处：《环境工程学报》2016年第7期3585-3590,共6页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(21207006);北京市科技新星资助项目(2008B25)

摘　　要：利用生物脱硫技术处理炼油厂含硫污水,硫化物选择性地氧化成单质硫。在进水硫化物605.3 mg·L^(-1)的条件下,硫化物去除率90.7%,单质硫生成率达到80.8%。提取反应器中的DNA样本,构建16S rDNA克隆文库对反应器中的生物群落进行分析。对51个阳性克隆子(长度约为1 400 bp)进行测序,测序结果在NCBI数据库利用Blast比对并构建系统发育树。测序结果表明,生物脱硫反应器细菌种群可分为10个类群,数量最多的是γ-变形菌(γ-Proteobacteria)占76.6%,其余类群共占23.4%。γ-Proteobacteria类群中数量最多的是具有脱硫功能的Halothiobacillus sp.(66.7%)、Thiovirga sp.(25.6%)以及Pseudomonas sp.(7.7%)。系统发育树分析结果表明,生物脱硫系统中形成了以化能自养型脱硫菌为主的微生物群落结构,异养菌数量较少。Sulfide in oil refinery wastewater was selectively oxidized to sulfur in a biological desulfurization reactor. When sulfide concentration in the influent was 605. 3 mg ·L^（-1）,90. 7% sulfide removal was achieved and 80. 8% of sulfide was converted into sulfur. DNA was extracted from the reactor to construct a 16 S rDNA gene library for the analysis of biological communities. Fifty-one clones were randomly selected and their partial16 S rDNA（ 1 400 bp） was sequenced. Sequencing results were analyzed using the BLAST algorithm at NCBI and a phylogenetic tree was constructed,identifying 10 bacterial groups. The dominant bacterial community in the reactor is γ-Proteobacteria,which account for 76. 6% of the sequences. The majority of γ-Proteobacteria belong to desulfurizing bacteria,such as Halothiobacillus sp.（ 66. 7%）,Thiovirga sp.（ 25. 6%） and Pseudomonas sp.（ 7. 7%）.The phylogenetic analysis demonstrates that the desulfurization system has evolved into a unique microbial community structure consisting mainly of chemoautotrophic desulfurizing bacteria,rather than heterotrophic bacteria.

关 键 词：生物脱硫 16S RDNA 含硫废水 

分 类 号：X742[环境科学与工程—环境工程]