铜绿假单胞菌NY3及突变株代谢油污泥同步产胞外聚合物的特性  

Characterizations of degradation of oil sludge and synchronous production of extracellular polymers by Pseudomonas aeruginosa NY3 and its mutants

作　　者：王小豆 聂麦茜[1] 聂红云[1] 冯凯强[1] 万博[1] 蒋欣[1] 吕天顺 张百忍[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境工程学报》2016年第7期3891-3897,共7页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51278405);陕西省国际科技合作重点项目(2012KW-25);2011榆林市产学研项目

摘　　要：油污泥属危险性固体废弃物,大量排放已成为石化企业可持续发展的障碍。利用铜绿假单胞菌NY3及突变株,将油污泥转化为生物质,并研究了其特性。研究表明,NY3、NB1D、NB2D及NB12DD均能降解油污泥中烷烃,但NY3、NB1D和NB2D菌对分子量较高烷烃(从C17~C32)的降解率比双突变菌NB12DD分别平均提高19.68%、28.76%和24.04%。铜绿假单胞菌NY3及突变株能直接将油污泥中烃类转化为胞外聚合物。与未加共代谢碳源相比,葡萄糖作为共代谢碳源时(培养液中表观油浓度达15 g·L^(-1)),培养120 h后,NY3、NB1D、NB2D、NB12DD菌胞外聚合物产量分别提高83.3%、79.8%、20.6%和62.9%,且碱性条件有利于各菌株高效降解油转化为生物质,pH为8~9时,其胞外聚合物产量均最高,分别达到8.1、4.34、4.94和7.15 g·L^(-1),野生菌NY3转化碳源为生物质转化率最高,为52.75%。结构和组成分析表明,胞外聚合物主要为糖蛋白,蛋白4.4%,总糖44.1%,蛋白与糖的连接方式为O-糖苷键链接,91.1%大分子粒径分布在33.0~716.9μm范围。Massive discharge of oil sludge（ a hazardous solid waste） has become an obstacle for the sustainable development of petrochemical enterprises. In this study,the decomposition and transformation of oil sludge into microbial extracellular polymers by Pseudomonas aeruginosa NY3 and its genetic mutants were characterized.Alkanes in oil sludge could be degraded by P. aeruginosa NY3 and its genetic mutants. The average degradation rates of high-molecular-weight alkanes（ from C17 to C32） by strain NY3,NB1D（ alk B1 mutant）,and NB2D（ alk B2 mutant） were 19. 68%,28. 76%,and 24. 04% higher than that of strain NB12DD（ a double alk B1 and alk B2 mutant）. During degradation,the four strains transformed the oil sludge into extracellular polymers. The oil sludge（ 15 g·L^（-1）） was the main carbon source,and glucose that was supplemented as a co-metabolic carbon improved the production of extracellular polymers by approximately 83. 3%,79. 8%,20. 6%,and 62. 9% during the 120 h incubation period for strain NY3,NB1 D,NB2D,and NB12 DD,respectively. For pH 8 to 9 and glucose as a co-metabolic carbon source,the highest yields of the extracellular polymer secreted by the strains were 8. 1,4. 34,4. 94,and 7. 15 g·L^（-1）,respectively. The transformation rate of the carbon source by the wild-type strain NY3 was 52. 75%. Additionally,the secreted polymer was composed of approximately 4. 4% proteins and 44. 1%sugars,which were connected by O-glycoside bonds. With respect to polymer molecular size,91. 1% were in the range of 33. 0 to 716. 9 μm.

关 键 词：铜绿假单胞菌NY3 基因突变株 油污泥 降解率 胞外聚合物 

分 类 号：X703.5[环境科学与工程—环境工程]