微流控芯片在植物细胞研究中的应用进展  

The Application Progress of Microfluidic Chips in Studying Plant Cells

作　　者：王伟轩[1,2] 孙静弈 刘伟娜[1] 厚凌宇[1] 玉王宁[1] 何湘伟[1] 谢响明[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]华北理工大学理学院,唐山063009

出　　处：《生物技术通报》2016年第6期19-29,共11页Biotechnology Bulletin

基　　金：中央高校基本科研业务费专项资金资助(BLX2013027);国家自然科学基金青年科学基金项目(31400085);北京林业大学"北京市大学生科学研究与创业行动计划"项目(201510022027)

摘　　要：植物细胞的传统分析方法是将植物细胞在土壤或者琼脂平板上生长,然后在温室或植物生长室内观察植物的表型。这种方法耗时耗力,且结果分辨率比较低。微流控芯片具有微型化、体积小和高通量等特点,且可在微米水平精确控制植物细胞生长的微环境。因此,能够降低实验成本,缩短实验时间,并且可以达到单细胞水平的分析和鉴定。首先介绍了微流控芯片的加工材料和制备方法,总结了用于植物细胞研究的微流控芯片,重点阐述了近年来微流控芯片在植物根、花粉管、原生质体和细胞壁动力学等植物细胞研究中的应用进展,并展望了微流控芯片在植物细胞研究的应用前景。Conventional methods of studying plant cells rely on growing plant cells in soil pots or agarose plates,followed by screening the plant phenotypes in traditional greenhouses and growth chambers. These methods usually need a large number of experiments,and suffer from low spatial resolution. While the microfluidic chips have many advantages,such as miniaturization,small volume consumption and high-throughput analysis,etc,moreover,it allows to precisely control the micro-environment of plant cells at micron-level. Therefore,it can reduce the cost and experimental time,and achieve in vitrosingle cell analysis and characterization. In this paper,the materials and manufacturing methods of the microfluidic chip were firstly introduced,then the recent microfluidic chips for studying plant cells were summarized,further mainly the application progress of microfluidic chips in plant root,pollen tube,protoplasts,and cell wall biomechanics were expounded,and finally the future research directions of microfluidic chips for plant cell were prospected.

关 键 词：微流控芯片 聚二甲基硅氧烷 植物细胞 应用进展 

分 类 号：Q942[生物学—植物学]