重组酶聚合酶扩增技术研究进展  

Research Progress on Recombinase Polymerase Amplification

作　　者：景志刚[1] 董浩[2] 狄栋栋[1] 田莉莉[1] 范伟兴[1] 

机构地区：[1]中国动物卫生与流行病学中心,青岛266032 [2]中国动物疫病预防控制中心,北京102600

出　　处：《生物技术通报》2016年第6期47-53,共7页Biotechnology Bulletin

摘　　要：重组酶聚合酶扩增(recombinase polymerase amplification,RPA)技术是一种新兴的核酸恒温扩增技术,具有灵敏度高、特异性强、反应快速等特点。利用RPA技术可以对核酸拷贝数进行绝对定量并且可以同时检测多个靶标核酸序列,结合侧流层析试纸条或荧光信号监测装置等简易实验设备即可直接观察检测结果。就RPA技术的原理、发展、技术特点及其近年来在体外诊断、病原检测等领域的研究进展作一综述,旨在为该技术的深入研究和应用提供参考。Recombinase Polymerase Amplification（RPA）is a recently developed isothermal amplification method that offers highly sensitive and specific DNA and RNA detection. Using RPA,the initial copy number of target nucleic acid sequence from different samples can be absolutely quantified,and multiple target nucleic acid sequences can also be detected simultaneously. Combining with less complicated device such as lateral flow strips or a sequence-specific fluorescent probe,the results can be observed directly. This article summarized the fundamental principles,continuous development and technical features of RPA. In addition,research and application progress of RPA in the field of in vitro diagnostic,pathogen detection and so forth were reviewed,aiming at providing guidance for further development and application of this method.

关 键 词：重组酶聚合酶扩增技术 核酸恒温扩增 核酸检测 

分 类 号：Q78[生物学—分子生物学]