海洋细菌L_1-9双抗菌株的定殖能力及其对黄瓜枯萎病的防治作用  

Colonization of Double-resistance Strain of Marine Bacterium L_1-9 and Its Biocontrol Effect on Fusarium Wilt of Cucumber

作　　者：王军强[1] 汪晶晶[1] 王琦[1] 马桂珍[1] 暴增海[1] 王淑芳[1] 周向红[1] 

机构地区：[1]淮海工学院化工学院,连云港222005

出　　处：《生物技术通报》2016年第6期193-198,共6页Biotechnology Bulletin

基　　金：江苏省自然科学基金项目(BK20141248);江苏省高校自然科学重大项目(12KJA210001);连云港市科技局农业攻关项目(CN1307);国家级大学生实践创新训练计划项目(G201411641105002);江苏省"十二五"高等学校水产类重点专业项目资助

摘　　要：采用抗生素标记法,对海洋多黏类芽孢杆菌L1-9菌株进行标记,抗性菌株L1-9Str,rif对链霉素和利福平的抗性浓度分别为160μg/m L和20μg/m L。双抗菌株L1-9Str,rif的抑菌特性及其对链霉素和利福平的抗性经多次传代仍比较稳定。盆栽试验表明,该双抗菌株能在黄瓜根部土壤及根组织、茎基部、子叶和真叶组织中定殖。菌株L1-9Str,rif在黄瓜外根际、根际和根表土壤及黄瓜组织中的定殖动态基本一致,初期黄瓜组织中L1-9Str,rif菌量较少,随着时间的延长,菌量逐渐增加,达到高峰后逐渐减少。菌株L1-9Str,rif在根表土壤中菌量最多(1.76×109 CFU/g),其次是根际土壤,外根际土壤中菌量较少;在黄瓜组织中,菌株L1-9Str,rif其在子叶中的定殖能力最强(5.63×104 CFU/g),其次是根和茎基部(0-2 cm);调查至第26 d时在根部土壤中的含菌量仍保持在稳定的水平,其中根表土壤中含菌量最高(2.41×107 CFU/g),在黄瓜组织样品中,子叶中的含菌量最高(4.15×104 CFU/g);温室防病实验结果表明,菌株L1-9和L1-9Str,rif菌株对黄瓜枯萎病具有良好的防治效果,不同时期防效均达70%以上。上述结果表明来自海洋的多黏类芽孢杆菌L1-9菌株能在黄瓜根部土壤及幼苗组织中定殖,是一株有潜力的黄瓜枯萎病生防菌株。The Paenibacillus polymyxastrain L1-9 of marine bacterium was labeled by antibiotic marker,and strain L1-9Str,rif showed the resistance to rifampicin and streptomycin at the concentrations of 160 μg/mL and 20 μg/mL,respectively. The antibacterial characters of double-resistance strain L1-9Str,rif and its resistance to rifampicin and streptomycin were still stable after 10 times subculturing. The pot experiment showed that L1-9Str,rif successfully colonized in cucumber rhizosphere soil,root tissue,stem base,cotyledon,and true leaves. The colonized dynamics of L1-9Str,rif in cucumber ecto-rhizosphere soil,rhizosphere and rhizoplane soil,and cucumber tissues were similar;the amount of L1-9Str,rif colonized in cucumber tissue was little in the early stage,but increased gradually along with the cucumber growth,and reached a peak,then decreased gradually. The amount of L1-9Str,rif was the most in rhizoplane soil（1.76×109 CFU/g）,the followed in the rhizosphere, while the least in ecto-rhizosphere soil. The detection of L1-9Str,rif in cucumber tissues showed that it was the most in cotyledons（5.63×104 CFU/g）, followed by its number in roots and stem bases（0 - 2 cm）. The amount of bacteria in the rhizosphere soil was still in a stable level at 26 d, and the quantity from rhizoplane soil was the highest with 2.41×107 CFU/g. The quantity of bacteria in the cotyledons got the highest of 4.15×104 CFU/g among cucumber tissue samples. The greenhouse test showed that both strain L1-9 and strain L1-9Str,rif had favorable control effects on fusarium wilt of cucumber with the control efficacy over 70% at different stages. The above results indicated that strain P. polymyxastrain L1-9 may colonize in cucumber rhizosphere soil and tissues,and present a great potential for the bio-control of fusarium wilt of cucumber.

关 键 词：海洋细菌 多黏类芽孢杆菌 抗生素标记 黄瓜枯萎病 定殖 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]