三株栅藻属藻株光合自养产油脂的比较研究  

Lipid Production from the Photoautotrophy of Three Strains of Scenedesmus Species

作　　者：陈传红 吴洪 李青 尹顺吉 罗少敬 崔春莉 

机构地区：[1]新奥科技发展有限公司煤基低碳能源国家重点实验室,廊坊065001

出　　处：《生物技术通报》2016年第6期238-243,共6页Biotechnology Bulletin

基　　金：国家"973"计划重点项目(2012CB723606)

摘　　要：通过柱式光生物反应器培养,比较研究三株栅藻(Scenedesmus sp简写S.sp、Scenedesmus deserticola、Scenedesmus dimorphus)的生长及油脂含量情况,得出3株藻生物量最高的是S.deserticola为0.48 g/(L·d);其次是S.sp为0.41 g/(L·d);S.dimorphus产量最低为0.35 g/(L·d)。且培养后期经尼罗红染色清楚可见油脂分布,其中S.deserticola总脂含量达到了干重的55.3%,总脂产量为0.29 g/(L·d);S.dimorphus为46.7%,0.18 g/(L·d);S.sp为43.6%,0.20 g/(L·d)。三株栅藻主要脂肪酸为C18和C16,占总脂肪酸组成的85%以上,符合生物柴油的生产要求。综合比较,S.deserticola是一株性能优良的产油藻株。The growth and the lipid content of 3 strains of Scenedesmus were compared by column photobioreactor,and it was concluded that the strain of producing the highest biomass was Scenedesmus deserticola with 0.48 g/（L·d）,followed by Scenedesmussp（S.sp）0.41 g/（L·d）,and the lowest by S. dimorphuswith 0.35 g/（L·d）. In the late culture stage,the lipid distribution was clearly visible by Nile red staining. The total lipid content and the lipid productivity of S. deserticola were 55.3 % of DCW and 0.29 g/（L·d）,respectively,followed by S. dimorphus in 46.7 % and 0.18 g/（L·d）,S.sp in 43.6 % and 0.20 g/（L·d）. The main fatty acid composition of 3 Scenedesmus were C18 and C16,accounting for over 85% of total fatty acids,satisfying the requirements of biodiesel production. Comprehensive comparison revealed that the S. deserticolawas a lipid-producing strain with high performance.

关 键 词：栅藻 生长 尼罗红 生物量 油脂 

分 类 号：Q949.2[生物学—植物学]