光合细菌混合菌群HAU-M1产氢动力学实验研究  

EXPERIMENTAL STUDY OF HYDROGEN PRODUCING KINETICS OF PHOTOSYNTHETIC BACTERIA MIXED CULTURE HAU-M

作　　者：王毅[1] 张川[1,2] 荆艳艳[1] 张全国[1] 路朝阳[1] 

机构地区：[1]河南农业大学农业部农村可再生能源新材料与装备重点实验室,郑州450002 [2]华北水利水电大学电力学院,郑州450011

出　　处：《太阳能学报》2016年第6期1547-1553,共7页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(50976029;U1504509);教育部博士点基金(20134105130001)

摘　　要：研究光合细菌混合菌群以葡萄糖为底物,光合产氢过程中的生长和产氢动力学特性,分析混合菌群利用葡萄糖产氢过程中的基质降解规律及代谢产物的生成规律。研究结果表明:光合细菌混合菌群以葡萄糖为底物产氢时,150 mmol/L的葡萄糖添加量是最佳添加浓度,产氢过程存在代谢产酸的过程,产氢高峰期葡萄糖主要代谢产物为乙酸,此时产气速率大;产氢末期葡萄糖主要代谢产物为丁酸,此时产气速率较低。建立基于Monod方程的光合细菌混合菌群产氢过程中的生长动力学模型,模型可较好地描述混合菌群产氢过程中生长延滞期和对数增长期菌体的生长变化规律,最大比生长速率μmax为0.214 h^(-1),饱和常数KS为8.257。建立光合细菌混合菌群产氢过程中的底物消耗动力学模型,模型可较好地描述混合菌群产氢过程中产氢延滞期和产氢高峰期的葡萄糖降解规律,细胞得率系数YX/S为0.352 g/mol,维持系数m为0.85。The dynamical characteristics of hydrogen production and cell growth of photosynthetic bacteria mixed culture HAU-M1 utilizing glucose as the substrate were studied, the propensity of substrate degradation and metabolite generation in the hydrogen production process were explored. The results show that 150 mmol/L of glucose is the best concentration for hydrogen production by photosynthetic bacteria mixed culture HAU-M1 utilizing glucose as the substrate, there is the metabolic acid production process in hydrogen production process, the major metabolite of glucose is acetic acid at hydrogen production peak, the hydrogen yield is larger at this period, the main metabolite of glucose is butyric acid at the end of hydrogen production with lower hydrogen yield. The growth dynamical model of photosynthetic bacteria mixed culture HAU-M1 in hydrogen production process has been set up based on Monod equation, which can better describe the growth and change rule of culture in growth lag phase and log growth phase in the hydrogen production process of mixed culture, the maximum specific growth rate μmax is 0.214 h-1, the saturation constant Ks is 8.257. The dynamical model of substrate consumption in hydrogen production process of photosynthetic bacteria mixed culture has been established, which can better describe the rules of H2 production lag phase in H2 production process of mixed culture and glucose degradation in H2 production peak phase, the cell yield coefficient YX/S is 0.352 g/mol and the maintain coefficient m is 0.85.

关 键 词：光合细菌混合菌群 混合菌群 产氢 HAU-Ml 动力学 MONOD方程 

分 类 号：TK511.4[动力工程及工程热物理—热能工程]