tuftsin衍生物T肽对小鼠T细胞存活和分化的影响  

Synthetic tuftsin analog polypeptide affects survival and differentiation of T cells

作　　者：安映红[1] 宣自学 徐城望 杨德宣[3] 韩苏[4] 袁守军[3] 

机构地区：[1]中国人民解放军空军总医院临床检验中心生化室,北京100142 [2]浙江省人民医院药学部,浙江杭州310014 [3]军事医学科学院放射与辐射医学研究所药理毒理室,北京100850 [4]南开大学药学院,天津300071

出　　处：《中国新药与临床杂志》2016年第6期441-444,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：重大新药创制国家科技重大专项综合新药研究开发技术大平台项目(2012ZX09301003-001);国家自然科学基金(30973564)

摘　　要：目的探讨tuftsin衍生物T肽(TP)对T细胞存活、分化的影响。方法无菌分离正常小鼠和荷瘤(B16-F10)小鼠胸腺细胞,分为正常T细胞组、正常T细胞+TP组、荷瘤T细胞组和荷瘤T细胞+TP组,TP终浓度为4.18μmol·L^(-1),处理72 h后收集培养的胸腺细胞,Guava ViaCount流式细胞术检测细胞存活率,Annexin V-FITC和PI检测细胞凋亡,CD4-FITC和CD8a-Per CP双标记检测细胞的分化成熟。结果正常T细胞组细胞存活率为(26.70±2.40)%,而加入TP后细胞存活率为(36.50±2.00)%,两组比较有显著差异(P<0.05);荷瘤T细胞+TP组细胞存活率为(20.70±2.20)%,与荷瘤T细胞组细胞存活率(17.80±1.30)%相比无显著差异(P>0.05)。正常T细胞组早期凋亡和晚期凋亡细胞百分比分别是(28.07±1.43)%和(8.45±0.48)%,加入TP后,早期凋亡和晚期凋亡细胞百分比分别是(15.90±0.45)%和(5.10±0.21)%,加药前后比较均有显著差异(P<0.01)。正常T细胞组CD4^+和CD8^+双阳细胞的比例为(70.50±1.91)%,经TP处理后CD4^+和CD8^+双阳细胞的比例降低为(55.30±3.81)%,比较有显著差异(P<0.01)。正常T细胞组CD4^+或CD8^+单阳细胞的比例分别为(22.13±1.12)%和(6.74±0.36)%,而经TP处理后CD4^+或CD8^+单阳细胞的比例分别为(34.87±2.77)%和(9.65±0.61)%,均显著增加(均P<0.01)。结论 TP能促进正常胸腺T细胞存活,抑制凋亡,并诱导其分化成熟。AIM To investigate the effects of synthetic tuftsin analog polypeptide （TP） on the survival and differentiation of T cells. METHODS Thymus cells were isolated from sterile mouse thymus, including normal and tumor-bearing mouse, and treated by TP （4.18 μmol·L^-1） for 72 hours. Thymocytes with different treatment were detected by Guava ViaCount flow cytometric for cell survival, annexin V- FITC and PI for apoptosis, CD4-FITC and CD8a-PerCP for cell differentiation and maturation. RESULTS Cultured for 3 days, thymocytes viability of normal mice was （26.70 ± 2.40） %, while the viability was （36.50 ± 2.00） % in TP treated thymocytes （P 〈 0.05）. The viability of thymocytes of B16-F10 mice was （17.80 ± 1.30） %, and that treated with TP was （20.70 ± 2.20） %. There was no statistical significance after TP treatment （P 〉 0.05）. The percentage of early and late thymocytes apoptosis in normal thymocytes was （28.07 ± 1.43） % and （8.45 ±0.48） %, respectively. And after TP treatment, the percentage of early and late thymocytes apoptosis was （15.90 ± 0.45） % and （5.10± 0.21） %, significant differences can be observed, respectively （P 〈 0.01）. The percentage of CD4^＋/CD8^＋ double-positive T cells in normal thymocytes was （70.50±1.91） %. And the percentage of CD4^＋/CD8^＋ double-positive T cells treated with TP was decreased as （55.30 ±3.81） % （P 〈 0.01）. CD4^＋ and CD8^＋ cells in separated primary thymocytes were （22.13 ± 1.12） % and （6.74 ± 0.36） %, respectively. After TP treated, the percentage of CD4^＋ and CD8^＋ were （34.87 ± 2.77） % and （9.65 ± 0.61） %, respectively （P 〈 0.01）. CONCLUSION TP can promote the survival of thymocytes, inhibit the apoptosis progress, and induce thymoeytes differentiation and maturation.

关 键 词：TUFTSIN 胸腺细胞 细胞分化 细胞凋亡 

分 类 号：R965.1[医药卫生—药理学]