下调Slit/Robo通路抑制兔血管成形术后血管再狭窄  

The study of inhibit the Slit / Robo pathway on vascular restenosis after angioplasty in rabbits

作　　者：曾智桓[1] 张仁丹[1] 赵艳群[1] 郑坚奕[1] 周万兴[1] 张卫[1] 朱桂平[1] 李博维[1] 

机构地区：[1]广东药科大学附属第一医院心血管内科,广州市510080

出　　处：《实用医学杂志》2016年第12期1934-1937,共4页The Journal of Practical Medicine

基　　金：广东省医学科研基金项目(编号:A2014359);广州市越秀区科信局基金项目(编号:2014-WS-034)

摘　　要：目的:探讨下调Slit/Robo通路对兔血管成形术后血管再狭窄的影响及机制。方法:将30只雄性新西兰大白兔分成3组,即空白组、对照组及实验组,每组10只。高脂饲养,除空白组外均制作髂动脉内皮剥脱血管狭窄模型。普通喂养4周后在血管狭窄部位行经皮球囊扩张成形术制作再狭窄模型。随后予R5抗体进行腹腔注射。继续喂养4周后再次血管造影检查,用图像工作站对血管造影结果行血管狭窄分析,并测定血清中Slit2、Robo1浓度,取髂动脉行HE染色。结果:成功建立兔髂动脉成形术后血管再狭窄动物模型。对照组及实验组与空白组相比,Slit2、Robo1血清浓度均显著升高(P<0.01),但实验组经R5抗体干预后,Slit2、Robo1血清浓度较对照组明显降低(P<0.05),经血管造影证实髂动脉血管面积狭窄率及直径狭窄率均降低(P<0.05)。结论:兔血管再狭窄模型Slit2/Robo1的表达显著增高,R5抗体可有效抑制Slit2/Robo1的表达,下调Slit2/Robo1信号通路可治疗兔血管成形术后再狭窄。Objective To study the effect and mechanism of down-regulating Silt2/Robo 1 signaling pathway on rabbit iliac artery after angioplasty restenosis. Methods The 30 male New Zealand white rabbits were divided randomly into 3 groups , namely the blank group , the control group , and the experimental group , 10 rabbits in each group. Hign-fat feeding , the rabbits were produced endothelial denudation of iliac artery stenosis model. Another 4 weeks of feeding , percutaneous balloon angioplasty was performed. Then R5 antibody was injected into the abdominal cavity. After 4 weeks of feeding ,angiography again. The results of angiography was analysied by image workstation. The concentrations of Slit2 and Robo1 was detected by ELISA. The iliac artery tissue examined by HE staining. Results The rabbit iliac artery after angioplasty restenosis animal model was set up successfully. Compared with the control group and the experimental group , the serum concentration of Slit2 and Robo1 were significantly higher （P 〈 0.01） than the blank group. But in the experimental group, the Slit2 and Robo1 serum concentrations were significantly lower than those in the control group （P 〈 0.05） after R5 antibody intervention. The area ratio stenosis and diameter stenosis rate of iliac artery were reduced that confirmed by angiography. Conclusion The expression of Slit2/Robo1 was significantly higher in the rabbit model of vascular restenosis. R5 antibody can effectively inhibit the expression of Slit2/Robo1. Down regulation of Slit2/Robo1 signaling pathway in the treatment of restenosis after angioplasty in rabbits.

关 键 词：SLIT2 ROBO1 R5抗体 血管成形术 再狭窄 

分 类 号：R541.4[医药卫生—心血管疾病]