微小 RNA-126在糖尿病视网膜病变患者血浆中的表达及作用机制研究  

Expressions of microRNA -126 in the blood plasma in patients with diabetic retinopathy and its mechanism

作　　者：王慧[1] 温晏[1] 闫丽[1] 于晓明[1] 

机构地区：[1]山东省交通医院眼科,山东省济南250031

出　　处：《中国基层医药》2016年第14期2134-2137,I0001,共5页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的：探讨微小 RNA-126（miR-126）在糖尿病视网膜病变（DR）患者血浆中的表达及可能的作用机制。方法选取 DR 患者81例、2型糖尿病患者65例和健康人群（对照组）50例，实时荧光定量 PCR 检测血浆中 miR-126表达，酶联免疫吸附试验（ELISA 法）检测血清中 VEGF 浓度，采用 Pearson 相关分析分析变量间关系，采用受试者工作特征曲线（ROC 曲线）分析 miR-126在预测 DR 发生中的价值。结果DR 患者和2型糖尿病患者血浆中 miR-126表达量均低于对照组，且 DR 患者低于2型糖尿病患者，DR 患者和2型糖尿病患者血清中 VEGF 浓度均高于对照组，且 DR 患者高于2型糖尿病患者，差异均有统计学意义（P ＜0．05）；Pearson 相关分析显示，DR 患者血浆中 miR-126表达量与 VEGF 浓度呈负相关（r ＝－0．352，P ＜0．05）；ROC曲线分析显示，患者血浆中 miR-126表达量在预测 DR 发生时曲线下面积0．848（95％CI：0．776～0．919），当miR-126表达量≤0．47时，灵敏度83．1％，特异度80．2％。结论DR 患者血浆 miR-126表达降低，而血清VEGF 水平升高，miR-126可能通过负性调节 VEGF 而调控视网膜血管新生，有望成为早期发现 DR 的血液指标。Objective To investigate the expression of microRNA -126（miR -126）in the blood plasma in patients with diabetic retinopathy （DR）and its possible mechanism.Methods 81 patients with DR,65 patients with type 2 diabetes and 50 healthy people （control group）were selected.The expression of miR -126 in plasma was detected by real -time PCR.The serum concentration of VEGF was detected by enzyme -linked immunosorbent assay （ELISA）.The correlations between variables were analyzed by Pearson correlation analysis.The value of miR -126 in＆amp;nbsp;predicting the occurrence of DR was analyzed by using receiver operating characteristic curve （ROC curves）.Results The expressions of miR -126 in plasma in DR patients and type 2 diabetes were lower than the control group,which in DR patients was lower than the type 2 diabetes,the serum concentrations of VEGF in DR patients and type 2 diabe-tes were higher than the control group,which in DR patients was higher than the type 2 diabetes,the differences were statistically significant （all P 〈0.05）.Pearson correlation analysis showed that the expression of miR -126 in plasma was negatively correlated with the concentration of VEGF （r =-0.352,P 〈0.05）.ROC curve analysis showed that the area under the curve for expression level of miR -126 in predicting DR occurrence was 0.848 （95% CI：0.776 -0.919）,when the expression level of miR -126 ≤0.47,the sensitivity was 83.1%,and specificity was 80.2%. Conclusion The expression of miR -126 in plasma in DR was reduced,while the serum level of VEGF was elevated.The miR -126 might through negatively regulated VEGF to regulate retinal angiogenesis.It expected to be blood indicators for early detecting DR.

关 键 词：糖尿病视网膜病变 MIR-126 血浆 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]