糖肝煎浓缩丸水提部分的提取工艺研究  

Study on Extraction Technology for Water Extract of Tangganjian Concentrated Pills

作　　者：吴莹[1] 张义生[2,3] 范彦博[2,3] 

机构地区：[1]湖北中医药大学,2014级研究生武汉430065 [2]国家中医药管理局中药制剂三级实验室 [3]武汉市中医医院

出　　处：《中国药师》2016年第7期1236-1238,共3页China Pharmacist

基　　金：湖北省中西医结合项目(编号:2013Z-Y25)

摘　　要：目的:优选糖肝煎浓缩丸水提部分的提取工艺参数。方法:以芍药苷含量和浸膏得率作为评价指标,芍药苷含量采用高效液相色谱法,色谱条件:色谱柱:Wonda Sil C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.15%磷酸溶液(16∶84);流速:1.0 ml·min-1;柱温:30℃;检测波长:230 nm。以加水量、煎煮时间和煎煮次数作为考察因素,采用正交设计法进行方差分析优选水提取工艺参数。结果:优选的提取工艺为加12倍量水,提取3次,每次1 h。结论:优选的提取工艺合理、稳定、可行,为糖肝煎浓缩丸提取工艺提供了试验依据。Objective：To optimize the extraction parameters for the water extract of Tangganjian concentrated pills .Methods：U-sing the content of paeoniflorin and extraction yield as the evaluation indices .An HPLC was used to determine the content of peoniflorin in the extract, and the chromatographic conditions were as follows： a WondaSil C18 chromatographic column （250 mm ×4.6 mm, 5μm）, the mobile phase was acetonitrile-0.1%phosphoric acid solution （16∶84） with a flow rate of 1 ml· min-1, the column tem-perature was 30℃and the detection wavelength was 230 nm.The amount of water , extraction time and extraction times were regarded as the influencing factors ,an orthogonal design was adopted to develop the analysis of variance for extraction parameters for water ex -tract.Results：The optimal extraction process was as follows：adding 12-fold amount of water and extracting 3 times with 1 h for each time.Conclusion：The optimum extraction process is reasonable , stable and feasible, which provides experimental basis for the extrac-tion process of Tangganjian concentrated pills .

关 键 词：糖肝煎浓缩丸 提取工艺 芍药苷 浸膏得率 正交设计 

分 类 号：R284.2[医药卫生—中药学]