黄柏提取物对大鼠心肌损伤的保护作用  

Protective Effect of Cortex Phellodendri Extract on Myocardial Injury in Rats

作　　者：齐梁煜 黄月维 黄莹[1] 李芸达[1] 李兆叠 零新岚 赵善民[1] 冯雪萍[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《中国药师》2016年第7期1259-1262,共4页China Pharmacist

基　　金：2014年国家级大学生创新创业训练计划项目(编号:201410599002);2014年自治区级大学生创新创业计划项目(编号:201410599021);右江民族医学院民族医药协会2014-2016年度科研创新立项项目(编号:20140615)

摘　　要：目的:探讨黄柏水提物和醇提物对垂体后叶素和盐酸异丙肾上腺素致大鼠心肌损伤的保护作用及其可能的作用机制。方法:将SD大鼠随机分为正常对照组、模型组、复方丹参片组、黄柏水提组、黄柏醇提组,建立垂体后叶素和盐酸异丙肾上腺素大鼠心肌损伤模型,检测血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)活性、心肌组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与正常对照组比较,垂体后叶素建立的大鼠心肌损伤模型组血清LDH活性、CK活性和心肌组织MDA含量明显升高,心肌组织SOD活性明显降低(P<0.01);盐酸异丙肾上腺素建立的大鼠心肌损伤模型组心肌组织MDA含量明显升高,心肌组织SOD活性明显降低(P<0.01)。与垂体后叶素建立的大鼠心肌损伤模型组相比较,各给药组血清LDH活性、CK活性和心肌组织MDA含量明显降低,心肌组织SOD活性明显升高,差异有统计学意义(P<0.05或P<0.01);与盐酸异丙肾上腺素建立的大鼠心肌损伤模型组相比较,各给药组心肌组织中MDA含量明显降低,SOD活性明显升高,差异有统计学意义(P<0.05或P<0.01)。结论:黄柏提取液对垂体后叶素和盐酸异丙肾上腺素引起的大鼠心肌损伤有一定保护作用。Objective：To investigate the protective effects and possible mechanisms of cortex phellodendri water extract and etha -nol extract on the myocardial injury induced by pituitrin and isoproterenol hydrochloride in rats .Methods：SD rats as the experimental animals were randomly divided into the normal control group , model group , compound Danshen tablets group , phellodendron water ex-tract group and phellodendron ethanol extract group .Pituitrin and isopropyl adrenaline hydrochloride were used to establish the myocar-dial injury model in rats.The serum CK, LDH activity, myocardial tissue SOD activity and MDA content were detected and compared . Results：Compared with those in the normal control group , the serum LDH activity , CK activity and MDA content were significantly in-creased , and the SOD activity in cardiac muscle and myocardial tissue was significantly decreased in the pituitrin -established myocardi-al injury model group （P〈0.01）.In the isopropyl adrenaline hydrochloride-established myocardial injury model group , the MDA con-tent in myocardial tissue was obviously increased , and the SOD activity in myocardial tissue was decreased obviously （P〈0.01）.The serum LDH activity, CK activity and MDA content were significantly decreased , and the SOD activity in cardiac muscle and myocardial tissue was increased significantly in all drug-taken groups when compared with those in the pituitrin-established myocardial injury model group, and the differences were statistically significant （P〈0.05 or P〈0.01）.The MDA content in myocardial tissue was significant-ly reduced , and the SOD activity was increased significantly in all drug-taken groups when compared with those in the isopropyl adrena-line hydrochloride-established myocardial injury model group , and the differences were statistically significant （P〈0.05 or P〈0.01）. Conclusion：Cortex phellodendri extract has certain protective effect on myocardial injury induced by pituitrin and isopropyl adrenaline hydrochloride in rats

关 键 词：黄柏提取物 垂体后叶素 盐酸异丙肾上腺素 心肌损伤 氧自由基 

分 类 号：R285.5[医药卫生—中药学]