HPLC法同时测定甘草酸二铵原料中18α-甘草酸和18β-甘草酸的含量  

Simultaneous Determination of 18α-Glycyrrhizic Acid and 18β-Glycyrrhizic Acid in Diammonium Glycyrrhizinate by HPLC

作　　者：张轶华 闫凯 赫晓军 姜建国 

机构地区：[1]河北省药品检验研究院,石家庄050011

出　　处：《中国药师》2016年第7期1397-1399,共3页China Pharmacist

基　　金：河北省科技支撑计划项目(编号:10276430)

摘　　要：目的：建立HPLC法同时测定甘草酸二铵原料中差向异构体18α-甘草酸和18β-甘草酸的含量。方法：色谱柱：Diamonsil C18柱（200 mm×4.6 mm,5μm）;流动相：水相（水-60%高氯酸溶液为48∶0.5,用氨水调节p H至8.0）-甲醇（48∶52）;检测波长：248 nm;流速：1.0 ml·min-1;柱温：30℃;进样量：20μl。结果：18α-甘草酸和18β-甘草酸得到良好分离;线性范围均为0.005 0~1.000 0 mg·ml-1（r分别为0.999 7和0.999 3）;平均回收率分别为99.7%和99.1%,RSD分别为0.9%和0.4%（n=9）。结论：该法专属性强,准确度高,可用作甘草酸二铵原料中差向异构体的含量测定。Objective：To establish an HPLC method for the simultaneous determination of 18α-glycyrrhizic acid and 18β-glycyr-rhizic acid in diammonium glycyrrhizinate .Methods：A Diamonsil C18 column （200 mm ×4.6 mm, 5 μm） was used with the mobile phase of water （water-60%perchloric acid solution：48∶0.5, adjusting pH to 8.0 with ammonium hydroxide）-methanol （48∶52）. The detection wavelength was set at 248 nm and the flow rate was 1.0 ml· min-1 .The column temperature was 30℃and the injection volume was 20 μl.Results：18α-Glycyrrhizic acid and 18β-glycyrrhizic acid were well separated .They had a good linear relationship within the range of 0.005 0-1.000 0 mg· ml-1（r=0.999 7 and 0.999 3）.The average recovery was 99.7%and 99.1%, and RSD was 0.9%and 0.4%, respectively （n=9）.Conclusion：The method is accurate, simple and reproducible, which can be used for the simultaneous determination of the two constituents in diammonium glycyrrhizinate .

关 键 词：甘草酸二铵 18Α-甘草酸 18Β-甘草酸 差向异构体 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]