内蒙古地区椎间盘退变患者ACE基因的多态性的研究  

Study the polymorphisms of ACE alleles in patient with disc degeneration in Inner Mongolia

作　　者：孙虹[1] 张玉华[1] 徐伟明[1] 崔明玉[1] 娜琴[1] 王静媛[1] 陈阿梅[1] 

机构地区：[1]内蒙古医科大学基础医学院,010059

出　　处：《中国优生与遗传杂志》2016年第7期20-21,共2页Chinese Journal of Birth Health & Heredity

基　　金：内蒙古自然科学基金资助项目(2013MS1191)

摘　　要：目的调查内蒙地区汉族椎间盘退变人群的ACE基因I/N的多态性,并同汉族健康人群比较,分析其遗传特点。方法应用ACE基因内含子16多态部位侧翼的前体进行PCR扩增。等位基因在琼脂胶上电泳,溴乙啶染色,紫外灯下观察。结果椎间盘退变人群的DD,DI和II基因型的频率是0.12,0.63和0.24、健康人群的DD,DI和II基因型的频率是0.33,0.32和0.35。结论在内蒙古地区汉族人群的研究中发现ACE基因I/N的多态性与椎间盘退变相关。Objective：To investigate the Insertion/Deletion（I/N）Polymorphism of angiotensin converting enzyme（ACE）in the patient with disc degeneration in inner Mongolia,and analysis their genetics characteristics. Methods：Genomic DNA was amplified by polymorphic region in intron 16 of the ACE gene. Allele were detected on agarose gel stained with ethidium bromide. Results：The frequencies of DD,DI and II genotype were 0.12,0.63 and 0.24 in disc degeneration and 0.33,0,32 and 0.35 in the control;respectively. Conclusion：Our study demonstrated that For in intron 16 of the ACE gene polymorphisms,DD,DIgenotype,allele I were found to be significantly associated with disc degeneration risk in Inner Mongolia in Chinese population.

关 键 词：椎间盘退变 ACE基因 多态性 

分 类 号：R681.53[医药卫生—骨科学]