阿托伐他汀的新效应:升高心肌细胞载脂蛋白J表达  

New effect of atorvastatin:augmenting Apo J expression in cardiomyocytes

作　　者：马彦卓[1] 孔令锋[1] 南凯 王冬梅[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院心内科,河北石家庄050000 [2]河北省卫生医学科技发展研究中心,河北石家庄050051

出　　处：《心脏杂志》2016年第4期411-414,共4页Chinese Heart Journal

摘　　要：目的观察阿托伐他汀对心肌细胞载脂蛋白(Apo)J的影响。方法分离大鼠乳鼠心肌细胞,并给予PBS、Ang II、阿托伐他汀和Apo J干预;细胞随机分为对照组;Ang II(0.1μmol/L)组;阿托伐他汀(10μmol/L)预处理1 h+Ang II组;Apo J(20μg/ml)预处理1 h+Ang II组;通过MTT检测法和Caspase-3/7活性检测试剂盒检测细胞活性和细胞凋亡变化;利用Western blot检测心肌细胞Apo J表达情况。结果与对照组相比,Ang II引起心肌细胞活性明显下降(P<0.01),凋亡细胞显著增加(P<0.01),心肌细胞Apo J表达下调(P<0.01)。阿托伐他汀预处理后,再给予Ang II干预,与Ang II处理组相比,心肌细胞活性明显增加(P<0.01),凋亡细胞显著减少(P<0.01),心肌细胞Apo J表达上调。给予外源性Apo J预处理,再给予Ang II干预,可抑制Ang II引起的细胞损伤,作用与阿托伐他汀类似。结论阿托伐他汀通过升高心肌细胞Apo J表达,减轻Ang II引起的细胞损伤。AIM To investigate whether atorvastatin suppresses cell injury induced by angiotensin Ⅱ （Ang Ⅱ） via enhancing apolipoprotein J （ApoJ） expression. METHODS Cultured neonatal rat ventricular myocytes （NRVMs） were incubated with AngII for 24 h or preincubated with atorvastatin or ApoJ for 1 h. Cell injury was analyzed by MTT and caspase-3/7 assay kits and ApoJ expressions were detected using Western blot analysis. RESULTS Ang Ⅱ significantly increased cell viability and decreased apoptotic cardiomyocytes. ApoJ expression was significantly reduced by Ang Ⅱ. These effects were abolished by atorvastatin. Moreover, ApoJ administration exerted similar effects on NRVMs as atorvastatin. CONCLUSION Atorvastatin may mediate cardioprotection against cell injury induced by Ang II via elevating ApoJ expressions.

关 键 词：阿托伐他汀 载脂蛋白J 血管紧张素II 凋亡 

分 类 号：R96[医药卫生—药理学]