用HPLC-ELSD法测定复方银灵通胶囊中银杏总内酯的含量  

Content determination of ginkgolides in compound Yinlingtong capsules by HPLC-ELSD method

作　　者：肖莹[1] 陈亮 黄杰[3] 马静洁[3] 钱一帆[2] 

机构地区：[1]中国药科大学理学院分析化学教研室,南京211198 [2]南京中科药业股份有限公司质量部,南京210008 [3]延边大学药学院药剂学教研室,吉林延吉133002

出　　处：《药学服务与研究》2016年第3期200-202,共3页Pharmaceutical Care and Research

摘　　要：目的:建立HPLC-ELSD法测定复方银灵通胶囊中银杏总内酯的含量。方法:采用Shim Pack VP-ODS C18色谱柱(150mm×4.6mm,5μm),柱温35℃,流动相为甲醇-四氢呋喃-水(25∶10∶65),流速1.0ml/min,用蒸发光散射检测器(ELSD)检测。结果:银杏内酯A、B、C和白果内酯在指定范围内线性关系良好,精密度RSD均<3%。测定银杏总内酯的重复性RSD为1.7%。样品溶液在配制后8h内稳定性较好,平均回收率为101.5%(RSD=1.5%,n=6)。6批样品中银杏总内酯的含量均>0.1%。结论:该方法准确、简便、可靠,分离效果好,可用于复方银灵通胶囊的质量评价。Objective： To establish a HPLC-ELSD method for content determination of ginkgolides in compound Yinlingtong capsules. Methods：The separation was performed on Shim Pack VP-ODS C18 column（150 mm× 4.6 mm,5μm） at the column temperature of 35 ℃. The mobile phase was composed of methanol-tetrahydrofuran-water（25 ： 10 ： 65） at a flow rate of 1.0 ml/min. Detection was made by using the evaporative light-scattering detector（ELSD）. Results： The contents of ginkgo lactone A,B,C and bilobalide were in good linearity with peak areas. The precision RSD were all less than 3%. The repetion RSD of ginkgolides determination was 1.7 %. Sample solution was stable within 8 hours after preparation and the average recovery rate was 101.5% （RSD= 1.5% ,n=6）. Contents of ginkgolides in 6 batches of samples were all more than 0.1%. Conclusion .. The method is accurate,simple, reliable, and could be used for quality control of compound Yinlingtong capsules. detector

关 键 词：复方银灵通胶囊 银杏总内酯 含量测定 色谱法 高效液相 蒸发光散射检测法 

分 类 号：R927.2[医药卫生—药学]