Microfluidic-based single cell trapping using a combination of stagnation point flow and physical barrier  

作　　者：Miao Yu Zongzheng Chen Cheng Xiang Bo Liu Handi Xie Kairong Qin 

机构地区：[1]Department of Biomedical Engineering, Faculty of ElectronicInformation and Electrical Engineering, Dalian University of Technology, Dalian 116024, China [2]Department of Electrical and Computer Engineering,National University of Singapore, Singapore 117576,Singapore [3]Western Reserve Academy, Hudson, OH 44236, USA

出　　处：《Acta Mechanica Sinica》2016年第3期422-429,共8页力学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grants 11172060 and 31370948)

摘　　要：Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier.The microfluidic device overcomes the weakness of the traditional ones, which have been only based upon either stagnation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addition, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system.It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and conveniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the relationship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier.The microfluidic device overcomes the weakness of the traditional ones, which have been only based upon either stagnation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addition, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system.It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and conveniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.

关 键 词：Single cell trapping Microfluidics Stagnation point flow Physical barrier Hydrodynamic tweezers Dynamic biochemical signal 

分 类 号：O35[理学—流体力学]