HPLC-ELSD同时测定银迪清胶囊中靛蓝和靛玉红含量  

Simultaneous Determination of Contents of Indigo and Indirubin in Yindiqing Capsules by HPLC-ELSD

作　　者：殷玥[1] 姜珍[2] 

机构地区：[1]长春医学高等专科学校药学系,吉林长春130031 [2]沈阳药科大学药学系,辽宁沈阳110016

出　　处：《中医药临床杂志》2016年第6期856-859,共4页Clinical Journal of Traditional Chinese Medicine

摘　　要：目的：建立同时测定银迪清胶囊中靛蓝和靛玉红含量的方法。方法：采用高效液相色谱—蒸发光散射检测法,色谱柱为KromasilC18柱（150mm×4.6mm,3.5μm）,流动相为乙腈-0.1%冰醋酸水溶液（52：48,v/v）,流速：1.0m L·min-1;ELSD参数：漂移管温度为110℃,空气流速为2.5L·min-1;进样量：20μL。结果：靛蓝质量浓度在350~3500μg·m L-1范围内,其自然对数与峰面积的自然对数成良好的线性关系（r=0.999 0）,平均回收率为100.3%（RSD=2.1,n=6）;靛玉红质量浓度在5~50μg·m L-1范围内,其自然对数与峰面积的自然对数成良好的线性关系（r=0.999 5）,平均回收率为99.4%（RSD=2.3,n=6）。结论：建立的方法简便、准确、重现性好,可用于银迪清胶囊的质量控制。Objective： To establish an method for simultaneous determination of contents of indigo and indirubin in Yindiqing capsules（traditional Chinese medicines）.Method： Indigo and Indirubin in Natural Indigo were determined by HPLC-ELSD; the operation was determined by Kromasil C 18 column（150 mm × 4.6 mm; 3.5 μm）; aeetonitril- 0.1% glacial acetic acid （（52： 48; v/v））was used as the mobile phase. The flow rate was 1.0 mL·min -1; ELSD parameters： the drift tube temperature is 110℃ ; the air velocity of 2.5 L·min -1; Sample quantity of 20μ L. Result： HPLC-ELSD was used to determine Indigo naturalis of indigo and indirubin in Yinxieqing capsules. The cabibration curve of indigo was linear within the range of 35- 350 μg·mL -1 （r = 0.999 0 ）. The recoveries of indigo were 100.3% （RSD = 2.1; n = 6）. The results show that the cabibration curve of indirubin was linear within the range of 5 - 50 μg·mL -1 （r = 0.999 5）. The recoveries of indirubin were 99.4% （RSD = 2.3; n = 6）. Conclusion： The analysis method is simple;accurate; reproducible with a strong specificity and be used for the quality control Yinxieqing capsules.

关 键 词：银迪清胶囊 靛蓝 靛玉红 高效液相色谱—蒸发光散射检测法 

分 类 号：R284.2[医药卫生—中药学]