一株耐镉假单胞杆菌的分离鉴定及其对镉的吸附研究  

Separation,Identification of a Cadmium-tolerant Pseudomonas and the Study of Its Adsorption Characters of Cd^(2+)

作　　者：陈雪姣[1] 郭雪玲[4] 南昊[2] 刘怡玮[2] 胡鑫[2] 张明科[3] 徐全乐[2] 

机构地区：[1]西北农林科技大学,林学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]西北农林科技大学医院,陕西杨凌712100 [4]河南安阳地区医院,河南安阳455000

出　　处：《湖北农业科学》2016年第11期2765-2768,2773,共5页Hubei Agricultural Sciences

基　　金：西北农林科技大学大学生创新性实验计划项目(201410712013);中央高校基本科研业务费项目(2014YB040);国家自然科学基金项目(31401910)

摘　　要：从污染水域筛选获得了1株可在含有100 mg/L Cd2+的培养基上生长的耐镉菌株Cd-t1。通过形态观察、16S r DNA扩增及系统发育分析,将其初步鉴定为嗜碱性假单胞菌(Pseudomonas alcaliphila)。不同Cd2+浓度处理下菌株的生长曲线显示,各浓度Cd2+处理延缓了该菌株的生长时期。进一步利用扫描电镜、能谱分析与质粒消除试验发现,该菌株的Cd2+耐受性与其形态适应性变化和C、N、O、P、Na、K、Ca等元素含量的改变相关,并决定于质粒上的镉耐受基因。A bacterial strain Cd-t1,which was capable of growing on the medium containing 100 mg / L Cd^（2＋）,was isolated from Cd^（2＋）contaminated water. It was identified as Pseudomonas alcaliphila preliminarily based on the morphological observation,16 S r DNA analysis and phylogenetic analysis. Growth curve of the strain in different Cd2 ＋concentrations showed that its growth period was delayed under these circumstances. Moreover, the results of SEM, EDS and plasmid elimination analysis showed that the Cd^（2＋）tolerance of the strain was related to the adaptive morphological alteration, chemical element content changing of C、N、O、P、Na、K、Ca, etc, and determined by Cd^（2＋）tolerance related gene that located in plasmid.

关 键 词：镉污染 假单胞杆菌 16S RDNA 能谱分析 扫描电子显微镜 

分 类 号：Q939.99[生物学—微生物学]