PEG6000模拟干旱对油茶幼苗形态结构的影响  

Effects of Drought Stress with PEG6000 on Morphology and Structure in Camellia oleifera seedlings

作　　者：张规富[1] 孙航远 

机构地区：[1]湖北科技学院核技术与化学生物学院,湖北咸宁437100

出　　处：《湖北农业科学》2016年第11期2829-2833,共5页Hubei Agricultural Sciences

基　　金：湖北省高等学校青年教师深入企业行动计划项目(XD2014273)

摘　　要：以二年生油茶(Camellia oleifera Abel.)幼苗为试验试材,Hogland营养液为水培底液,用聚乙二醇6000(PEG6000)人工模拟干旱胁迫环境,设置不同浓度(m/V)的PEG6000(5%、10%、15%、20%)对油茶幼苗进行水分胁迫处理,研究油茶幼苗在干旱胁迫下根系形态及叶片解剖结构的变化及适应性机制。结果表明,随着干旱胁迫的增强,油茶幼苗根粗呈现逐渐减小的趋势,根冠比则明显提高。5%、10%、15%的PEG6000干旱胁迫使油茶幼苗根体积增大以及最长根长降低,而20%的PEG6000干旱胁迫则使根体积与最长根长显著增加。随着干旱胁迫的增强,油茶幼苗叶片逐渐变薄,上表皮、下表皮、栅栏组织、海绵组织等厚度均呈下降趋势,栅海比逐渐升高。Two years old Camellia oleifera seedlings were used as experimental material,using Hogland nutrient solution for water base solution,and polyethylene glycol 6000（PEG6000） was selected to simulate drought stress environment,C. oleifera seedlings were treated with different concentrations（m / V） of PEG6000（5%,10%,15%,20%） nutrient solution, the root morphology, the leaf anatomical structure and adaptability mechanism of C. oleifera seedlings were studied under drought stress. The results showed that with the increase of drought stress,the root diameter of C. oleifera seedlings decreased,root shoot ratio increased significantly. 5%,10%,15% PEG6000 drought stress increased root volume and decreased maximum root length,while 20% PEG6000 drought stress increased root volume and maximum root length significantly. With the increase of drought stress,the leaf of C. oleifera seedling gradually thinned,the thickness of upper epidermis,lower epidermis,palisade tissue,sponge tissue showed a downward trend,and cell thickness rate increased gradually.

关 键 词：油茶(Camellia oleifera Abel.) PEG6000 根系形态 叶片解剖结构 

分 类 号：S794.4[农业科学—林木遗传育种]