基于氮掺杂多孔碳的乙酰胆碱酯酶传感器检测残留农药甲拌磷  

Detection of Phorate Using Acetylcholinesterase Biosensor Based on Nitrogen-doped Porous Carbon

作　　者：吴建宏[1] 吕冰婧 卫伟[2] 

机构地区：[1]华东石油技师学院,江苏扬州225129 [2]东南大学化学化工学院,江苏南京211189

出　　处：《分析测试学报》2016年第7期897-900,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21475020)

摘　　要：以制备的新型氮掺杂多孔碳为载体,玻碳电极（GC）为工作电极,构建了乙酰胆碱酯酶（ACh E）传感器,用于有机磷农药甲拌磷的检测研究。在1.0 mmol/L底物氯化乙酰胆碱溶液中的差分脉冲扫描结果表明,ACh E/GC电极上的峰电流为0.195 8μA,而ACh E/氮掺杂多孔碳/GC电极上的峰电流为0.841 4μA,说明氮掺杂多孔碳材料可有效固定ACh E,提高检测灵敏度。采用ACh E/氮掺杂多孔碳/GC电极对不同浓度甲拌磷进行测定,在6.0×10-10～1.2×10-6g/L浓度范围内,抑制率与甲拌磷浓度的负对数呈良好的线性关系（r2=0.998 5）。按照抑制率为10%计算,检出限为5.8×10-12g/L。采用加标回收法检测菠菜汁样品中的甲拌磷,回收率为91.7%～97.4%。Nitrogen-doped porous carbon was used to immobilize acetylcholinesterase（ACHE） to fab- ricate biosensor for the detection of organophosphorus pesticide phorate. The peak current was 0. 195 8 μA at the AChE/GC electrode, while 0. 841 4 μA was obtained at AChE/nitrogen-doped porous carbon/GC electrode, indicating that nitrogen-doped porous carbon can effectively immobilize AChE and enhance the sensitivity. The inhibition rate versus logarithm of phorate were linear in the range of 6. 0×10-10 -1.2×10-6 g/L, with a correlation coefficient（ r2） of 0. 998 5. The detection limit of phorate, calculated by inhibition rate of 10% , was 5.8 × 10-12 g/L. The sensor could be used for the detection of phorate in spinach juice with recoveries of 91.7% -97.4%.

关 键 词：甲拌磷 氮掺杂多孔碳 乙酰胆碱酯酶 氯化乙酰胆碱 

分 类 号：O657.1[理学—分析化学] F767.2[理学—化学]