PHBHHx与人脐带间充质干细胞的体内异位成骨研究  

Heterotopic osteogenesis in vivo of PHBHHx and human umbilical cord mesenchymal stem cells

作　　者：马敏先[1] 何志旭[2] 王梅 叶川[4] 王永[1] 曾筱[1] 张俊标 刘琴[1] 

机构地区：[1]贵州医科大学附属医院口腔修复科,贵阳550004 [2]贵州医科大学干细胞与组织工程中心,贵阳550004 [3]贵州省贵阳市口腔医院口腔修复科,550002 [4]贵州医科大学附属医院骨科,贵阳550004

出　　处：《重庆医学》2016年第20期2740-2743,2747,共5页Chongqing medicine

基　　金：贵州省科学技术基金资助项目[黔科合J字(2010)2177号];贵州省科学技术联合基金资助项目[黔科合LG字(2012)002号]

摘　　要：目的探讨以3-羟基丁酸-3-羟基己酸共聚酯(PHBHHx)为支架材料,以人脐带间充质干细胞(hUCMSCs)为种子细胞的复合物体内构建组织工程骨的能力。方法将hUCMSCs接种在PHBHHx上体外成骨诱导培养2周,诱导组为实验组,不滴加hUCMSCs组为对照组,植入裸鼠皮下,并分别于1、3、5个月取材行HE、Ⅰ型胶原免疫组织化学、碱性磷酸酶染色及逆转录聚合酶链反应(RT-PCR)检测。结果 PHBHHx表现出良好的细胞吸附性。实验组细胞大、小形态基本保持原状,成骨特异性指标检测均为阳性;对照组细胞则不能保持原状,体积逐渐缩小至完全降解,成骨特异性指标均为阴性。结论 PHBHHx复合hUCMSCs经体外成骨诱导后具有在裸鼠体内异位构建组织工程骨的能力。Objective To explore the ability for constructing tissue engineering bone in vivo in complex scaffolds with PHB HHx as the scaffolds material and buman umbilical cord mensenchymal stem ceils （hUCMCs） as the seed cells. Methods hUCM SCs were inoculated into PHBHHx scaffolds to induce osteogenesis culture in vivo for two weeks, the the induced group was the experimental group and those without instilling hUCMCs served as the control group, the nude mouse subcutaneous implantation was performed. Then taking material at 1,3,5 months after implantation in vivo was performed for conducting HE, collagen 1 immunohistochemical,alkaline phosphatase staining and RT-PCR. Results hUCMSCs showed good cellular adsorbability. The size and form in the experimental group basically maintained the original status,and the osteogenesis specific indicators were positive; but the control group did not keek the original status,its volume was gradually shrunk until complete degradation,and the osteogen- esis specific indicators were negative. Conclusion The PHBHHx scaffolds combined with hUCMSCs has the capability of Jn vivo heterotopic constructing tissue engineering bone in nude mouse by in vitro osteogenic induction.

关 键 词：间充质干细胞 组织工程 骨 3-羟基丁酸-3-羟基己酸共聚酯 

分 类 号：R783[医药卫生—口腔医学]