NIH3T3细胞中miR-29b表达变化对mPer1和Egr2的影响  

The effects of miR-29b expression change on mPer1 and Egr2 in NIH3T3 cells

作　　者：徐涛[1] 后望[1] 汪宇辉[1] 成姝婷[1] 刘延友[1] 江舟[1] 肖静[1] 郭慧玲[1] 王正荣[1] 

机构地区：[1]四川大学华西基础医学与法医学院.卫生部时间生物学重点实验室,四川成都610041

出　　处：《西部医学》2016年第7期900-903,共4页Medical Journal of West China

基　　金：国家自然科学基金(31371180);国家自然科学青年基金(3150035)

摘　　要：目的探讨NIH3T3细胞中miR-29b对血清诱导的立早基因Egr2 mRNA表达的影响及其机制。方法将体外培养NIH3T3细胞分为实验组、阴性对照组和空白对照组。实验组用miR-29b转染,阴性对照组空转,而空白对照组则不做任何处理。RT-qPCR检测近日节律基因mPer1与立早基因Egr2 mRNA的表达水平。结果实验结果显示,mPer1和Egr2的表达均发生了较明显的变化。与阴性对照组相比,空白对照组中mPer1和Egr2的mRNA表达水平无明显差异;而实验组中mPer1的mRNA表达降低了42%,Egr2的mRNA表达升高了49%。结论 miR-29b能上调Egr2的表达,其途径可能是通过抑制NIH3T3细胞近日节律基因mPer1的表达实现。Objective To investigate the effects of miR-29b on serum-induced immediate early gene Egr2 mRNA expression in NIH3T3 cells. Methods NIH3T3 cells cultured in vitro were divided into experiment group, negative con- trol group and blank control group. Experimental group transfected with miR-29b, negative control group idling, while the control group received no treatment. The expression of roPer1 and Egr2 were detected by RT-qPCR. Results The experimental results show that mPerl and Egr2 expressions were more obvious changes. Compared with the negative con- trol group, the mRNA expression level of mPerl and Egr2 were not obvious difference in the blank control group but, the mRNA expression of mPerl was reduced 42%, the mRNA expression of Egr2 was increased 49% in the experimental group. Conclusion miR-29b can increase Egr2 expression, the approach is probably achieved by inhibiting circadian gene mPerl expression in NIH3T3 cells.

关 键 词：mPer1 miR-29b Egr2 NIH3T3细胞 

分 类 号：R392[医药卫生—免疫学] R393[医药卫生—基础医学]