柯里拉京对小鼠单纯疱疹病毒性脑炎脑组织TLR9/MyD88通路影响  

Effects of Corilagin on TLR9/MyD88 Pathway of Brain Tissue in Mice Infected HSV-1

作　　者：周静静[1] 张清安[1] 陈斌[1] 梅元武[2] 

机构地区：[1]荆门市第一人民医院,湖北荆门448000 [2]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《辽宁中医药大学学报》2016年第7期53-56,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：湖北省卫生厅科研基金资助课题(2007JX3B54)

摘　　要：目的:研究小鼠模型脑组织感染后柯里拉京对HSV-1TLR9、My D88蛋白的表达影响。方法:通过注射的办法使得HSV-1侵入到小鼠脑组织内制造小鼠单纯疱疹病毒性脑炎的模型,40只小鼠随机分成正常组和单纯疱疹病毒组,每组20只。单纯疱疹病毒组中随机分为4组:空白对照组、柯里拉京组、HSV-1+生理盐水组、HSV-1+柯里拉京组,每组5只。Western Blotting检测各组小鼠脑组织TLR9、My D88蛋白的表达。各组小鼠脑组织TNF-α分泌的变化通过ELISA法分析检测。H-E染色切片观察脑组织病理变化。结果:(1)在小鼠发病高峰(通常是HSV-1病毒入侵脑组织后第5天),HSV-1+柯里拉京组与空白对照组相比,脑组织神经变性坏死程度轻,细胞水肿消退,变性坏死组织周围淋巴细胞浸润减少。(2)HSV-1+柯里拉京组与HSV-1+生理盐水组相比,TLR9、My D88 m RNA的表达降低有统计学差异[(9.36±1.02)Kb vs(14.84±1.83)Kb,(7.5±1.04)Kb vs(11.7±2.63)Kb,P<0.05]。(3)ELISE法检测各脑组织TNF-α分泌均有增加,HSV-1+柯里拉京组、HSV-1+生理盐水组TNF-α的分泌[(543.97±18.07)pg/m L、(1036.62±38.84)pg/m L]高于空白对照组和柯里拉京组,差异有统计学意义(P<0.05)。HSV-1+柯里拉京组与HSV-1+生理盐水组相比TNF-α降低有统计学差异(P<0.05)。结论:柯里拉京可通过TLR9/My D88通路的作用,降低TLR9、My D88 m RNA的表达,减少TNF-α的分泌,一定程度减轻HSE的炎性因子的产生。Objective ： Through the detection of TLR9, MyD88 protein expression and TNF- ct secretion of HSE murine model, to investigate the effect of corilagin on the HSE prognosis. Methods： Injection approach makes HSV-1 from entering into the manufacture of mouse brain tissue of mice with herpes simplex virus encephalitis model, 40 mice were randomly divided into normal group and herpes simplex virus groups of 20. Herpes simplex virus group were randomly divided into four groups ： control group, corilagin group, HSV-1 ＋ saline group, HSV-1 ＋ eorilagin groups of five. Western Blotting to detect the brain tissue of mice TLR9, MyD88 protein. Each brain tissue of mice TNF-α secretion by ELISA analysis to detect changes. H-E stained sections of brain tissue pathological changes observed. Results ： （ 1 ） After herpes simplex virus type 1 （ HSV-1 ） infection （ 5 days after the virus injection ）, the lesions of hemorrhage and necrosis in mice＇s brains could be observed by microscope, and the injury in much tess serious in corilagin group compared to the HSV-1 group. （ 2 ） Compared to HSV-I＋PBS group, HSV- 1＋corilagin decrease the TLR9, MyD88 mRNA expression. Compared to HSV-1＋PBS group, HSV-1＋corilagin decrease the TLR9, MyD88 mRNA expression[ （ 9.36±1.02 ） Kb vs （ 14.84±1.83 ） Kb, （ 7.5±1.04 ） Kb vs （ 11.7±2.63 ） Kb, P〈0.05]. （ 3 ） Compared to HSV-1＋PBS group, HSV-l＋corilagin decrease the secretion of TNF-α[ （ 543.97 ± 18.07 ）pg/mL vs （ 1036.62 ±38.84 ）pg/mL, P〉0.05]. Conclusions ： Corilagin treating HSE may decrease the secretion of TNF-α through reducing the TLR9/ MyD88 signaling pathway.

关 键 词：单纯疱疹病毒性脑炎 柯里拉京 肿瘤坏死因子-Α 

分 类 号：R512.3[医药卫生—内科学]