牛血红蛋白催化香豆素体系荧光光度法测定过氧化氢  

Fluorometric determination of hydrogen peroxide by bovine hemoglobin catalytic coumarin system

作　　者：单雅琦[1] 陈永宁[1] 张容珲 唐宁莉[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《桂林理工大学学报》2016年第2期333-336,共4页Journal of Guilin University of Technology

基　　金：国家自然科学基金项目(21165008)

摘　　要：在pH 3.5的NaOH-H_2SO_4缓冲溶液中,牛血红蛋白催化H2O2氧化香豆素生成7-羟基香豆素,导致体系的荧光强度明显增强。该氧化产物在456 nm处有最大荧光强度。实验表明:在456 nm处,体系的荧光增加值ΔF与H-2O_2的浓度在3.940×10^(-7)~7.880×10^(-5)mol/L范围内呈良好的线性关系,相关系数r为0.998 5,检出限为1.25×10^(-8)mol/L。据此建立了一种简单、灵敏度高的检测水样中痕量H_2O_2的荧光新方法。该方法已用于雨水及消毒液中H_2O_2的测定,结果满意。In pH 3.5 NaOH- H2SO4 buffer solution,with bovine hemoglobin as catalyst,coumarin was oxidized to produce 7-hydroxycoumarin by hydrogen peroxide,which had strong fluorescence in acid solution. At the length of maximum emission 456 nm,the increased value of fluorescence intensity（ ΔF） was linear to the concentration of hydrogen peroxide in the range of 3. 940 × 10^-7- 7. 880 × 10^-5mol / L,with a correlation coefficient of 0. 998 5 and a detection limit of 1. 25 × 10^-8mol / L. Based on this,a simple and highly sensitive fluorometric method was proposed for the determination of trace H2O2. This new fluorometric method can be applied to the analysis of H2O2 in rainwater and disinfectant fluid samples with satisfactory results.

关 键 词：过氧化氢 牛血红蛋白 香豆素 荧光法 

分 类 号：O657.3[理学—分析化学]