紫苏RAS基因启动子的克隆及序列分析  

Molecular Cloning and Sequence Analysis of RAS Gene Promoter of Perilla frutescens

作　　者：郝磊[1] 张泽生[1] 吕晓玲[1] 郭宇[1] 孙敏[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《湖北农业科学》2016年第10期2656-2660,共5页Hubei Agricultural Sciences

摘　　要：迷迭香酸合成酶(Rosmarinic acid synthase,RAS)是紫苏(Perilla frutescens)迷迭香酸生物合成途径中的关键酶,采用基因组步移方法,克隆得到长度为2 022 bp的紫苏RAS基因5′端的启动子片段,并对启动子序列的转录起始位点和顺式作用元件进行分析。结果表明,紫苏RAS基因启动子调控区域除了含有基本的TATA-box和CAAT-box元件外,还含有多个与植物胁迫和生长相关的顺式作用元件,如脱落酸、茉莉酸甲酯和赤霉素响应的顺式作用元件等,另外还包含多个光响应顺式作用元件。The rosmarinic acid synthase（RAS） is considered to be a key enzyme involved in rosmarinic acid biosynthesis pathway of Perilla frutescens. A 2 022 bp RAS gene promoter fragment was cloned using genomic walking technique. The analysis of transcription start point and cis-acting elements of the promoter showed that the fragment contained the conserved promoter sequence, such as TATA-box,CAAT-box. Furthermore,it contained many plant stress and growth-related cis-acting elements,such as cis-acting element involved in the ABA,Me JA and GA responsiveness,and it has many light responsive elements as well.

关 键 词：紫苏(Perilla frutescens) 迷迭香酸合成酶 启动子 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]