ZnS:Mn纳米晶的制备及其与羊抗兔抗体偶联的研究  

Synthesis of ZnS:Mn Semiconductor Nanocrystals and Their Coupling with Goat Anti-Rabbit IgG

作　　者：张翔[1] 

机构地区：[1]中国海洋大学材料科学与工程研究院,山东青岛266100

出　　处：《山东化工》2016年第13期8-10,12,共4页Shandong Chemical Industry

基　　金：国家自然科学基金(51172218)

摘　　要：利用金属有机相法合成了高质量的Zn S:Mn纳米晶,经过配体交换,成功将纳米晶溶于PBS缓冲液中。进一步利用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基硫代琥珀酰亚胺(NHS)使纳米晶表面的羧基活化,与羊抗兔抗体中的氨基形成酰胺键,成功将纳米晶与抗体偶联,最终的偶联产物保持了较高的荧光性能并具有低细胞毒性,满足高质量荧光生物探针的要求。In this article,High quality Mn2 ＋doped Zn S nanocrystals（Zn S：Mn） were successfully synthesized via an organometallic approach.A ligand exchange reaction had been introduced to give the Zn S：Mn nanocrystals hydrophilic surfaces and the obtained nanocrystals could disperse homogeneously in PBS buffer.Activated by 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）,the surface carboxyl groups of nanocrystals reacted with the amino groups in Ig G to form amide bonds and thus Zn S quantum dots were coupled with goat anti-rabbit Ig G successfully.The obtained QDs-Ig G conjugates were suitable for biological labeling and fluorescence imaging due to their high PL intensity and low cytotoxicity.

关 键 词：半导体纳米晶 ZN S 掺杂 生物应用 

分 类 号：O649[理学—物理化学]