金黄色葡萄球菌植入总量对兔桡骨骨髓炎模型的影响  

Effect of the total implantation amount of Staphylococcus aureus in rabbit model of bone osteomyelitis

作　　者：王晓波[1] 郑欣[1] 陈一心[1] 邱旭升[1] 朱彦丞 桂雪洋 王骏飞[1] 施鸿飞[1] 熊进[1] 

机构地区：[1]南京大学医学院附属鼓楼医院骨科,江苏210008

出　　处：《创伤外科杂志》2016年第7期415-419,共5页Journal of Traumatic Surgery

基　　金：国家自然科学基金项目(81401793);江苏省六大人才高峰项目(2012-WS-092);南京市卫生局项目(ZKX12026;YKK13079);江苏省研究生科研创新项目(省助SJZZ_0030)

摘　　要：目的通过不同量金黄色葡萄球菌植入兔桡骨,观察影像学及组织学变化,探讨兔桡骨骨髓炎动物模型中金黄色葡萄球菌的合适剂量。方法 35只成年雄性新西兰兔双侧桡骨制造骨窗,注入不同量(1×104~1×109CFU)的金黄色葡萄球菌作为实验组(A^F组),对照组注入0.1m L生理盐水,每组5只。分别于术后当日、术后2周、4周行X线及CT扫描检查。术后4周完成影像学评分后,处死兔取出桡骨完成肉眼观察评分,再作细菌培养、组织病理学检查并完成组织学评分。结果 F组兔因感染过重死亡2只;C、D、E、F组术后4周体重明显减轻,平均减轻量分别为(75.6±24.9)g、(119.0±42.7)g、(184.1±51.7)g、(194.9±63.2)g,体重改变组间无明显差异(P>0.05),切口渗出较多,可见窦道形成。术前术后体重变化、肉眼观察评分、影像学评分和组织学评分LSD法比较,P<0.05。术后4周影像学、肉眼观察及组织学检查D组只可见少量的骨感染表现,而E、F组可见严重的骨感染表现;E组及F组的肉眼观察评分[(2.6±0.2)和(2.8±0.1)]、影像学评分[(17.6±0.3)和(19.4±0.2)]和组织学评分[(7.2±1.1)和(8.4±0.2)]明显高于其他五组(P<0.05);细菌学检查仅D、E、F三组培养出细菌。结论兔桡骨急性骨髓炎动物模型制作的最佳细菌量为1×108CFU,本实验的方法简单易行,适用于建立骨髓炎动物模型。Objective Through implantation of different doses of Staphylococcus aureus into radial bone in rabbits,to observe the radiographic and microbial changes and to discuss an appropriate dose of Staphylococcus aureus in rabbit model of bone osteomyelitis. Methods Bone defects were made bilaterally in the radius of 35 male New Zealand rabbits. Different doses of staphylococcus aureus were inoculated as the experimental group A-F（ 1 ×10^4CFU for group A,1 × 105 CFU for group B,1 × 10^6 CFU for group C,1 × 107 CFU for group D,1 × 10^8 CFU for group E,and 1 × 10^9 CFU for group F）. 0. 1m L saline solution was injected into each rabbit in the control group（ n= 5）. X-ray and CT were taken immediately after surgery,2-week,and 4-week postoperatively. The radiographic score,visual inspective score and histological score were estimated four weeks postoperatively. The rabbits were sacrificed four weeks after surgery and the specimens were harvested for pathologic analysis and bacterial cultivation.Results Anorexia and lassitude were observed in all rabbits. In group F,two rabbits died from infection and thus were excluded from analysis. Body weight loss was observed in group C [（ 75. 6 ± 24. 9） g],group D [（ 119. 0 ±42. 7） g],group E [（ 184. 1 ± 51. 7） g],and group F [（ 194. 9 ± 63. 2） g],but there was no significant statistical difference in body weight change in these groups（ P〉0. 05）. LSD comparison showed that there were statistical differences in body weight,radiographic score,visual inspection score and histological score between preoperatively and postoperatively（ P〈0. 05）. The radiographic,visual inspective and histological appearance of osteomyelitis were evident in group E and F,but mild in group D. The radiographic score,visual inspection score and histological score were significantly higher in group E（ 2. 6 ± 0. 2,17. 6 ± 0. 3 and 7. 2 ± 1. 1） and group F（ 2. 8 ± 0. 1,19. 4 ±0. 2 and 8. 4 ± 0. 2） than those of other groups（ P〈0. 05）. Bacteria culture

关 键 词：桡骨 骨髓炎 骨感染 细菌 模型 兔 

分 类 号：R63[医药卫生—外科学]