西黄丸中总氨基酸含量测定方法研究  

Research on Method for Determination of Total Amino Acid in Xihuang Pills

作　　者：于双慧[1] 朱梦露 甄绮雯 冯锦英[2] 佟玲[3] 刘晓琳[3] 张蕾[2] 

机构地区：[1]沈阳市化工学校,辽宁沈阳110122 [2]广州中医药大学,广东广州510006 [3]天津天士力集团研究院,天津300410

出　　处：《亚太传统医药》2016年第14期46-48,共3页Asia-Pacific Traditional Medicine

基　　金：辽宁省青年科研骨干(基础教育)专项资助项目(JGZXQEB002)

摘　　要：目的：建立茚三酮比色法测定西黄丸中总游离氨基酸含量的方法。方法：以甘氨酸为对照品,采用茚三酮显色体系,以分光光度法测定西黄丸中总游离氨基酸的含量。结果：茚三酮比色法最佳反应条件为加入缓冲液的pH值为6.5,用量为1.0mL;显色剂用量为1.0mL;反应条件为100℃水浴加热20min。甘氨酸在2.40~12.0μg/mL浓度范围内与其吸光度呈良好的线性关系,标准曲线回归方程Y=202.1X-0.389 4,r=0.999 8。平均回收率为104.7%（n=6）,RSD=3.7%。结论：采用茚三酮比色法测定西黄丸中总游离氨基酸含量准确、灵敏、重复性好,适合推广应用。Objective：To establish the method for quantification of the total amino acid content in Xihuang Wan. Methods：The content of the total amino acid was determined by the ninhydrin colorimetry with glycine as the reference, and the established nin- hydrin colorimetry was systematically optimized by evaluating various condition factors of the reaction. Results： The optimal conditions was as following., pH value of buffer solution used in the test was 6.5 with the volume of 1.0mL,the volume of ninhydrin rea- gent was of 1.0mL,the reaction was performed in 100℃ water bath for 20min. A good linear relationship for the calibration curve was in the range of 2.40 - 12.0μg/mL, and the standard curve equation was Y： 202.1 X-0. 389 4, r： 0.999 8. The average recovery was 104.7 % with RSD being of 3.7 % （n= 6）. Conclusion ： The method was accurate, sensitive and reproducible, and characteristic of simplicity and practicability.

关 键 词：西黄丸 总氨基酸 甘氨酸 茚三酮显色反应 比色法 

分 类 号：R284.1[医药卫生—中药学]