文心兰切花花被不同开放阶段酶活性的变化  

Changes in Enzyme Activities of Perianth of Cut-flower Oncidium Orchid at Different Opening Stages

作　　者：黄浩宇[1] 肖云骁 石乐松 田晓岩[1] 刘进平[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室/农学院,海口570228

出　　处：《热带生物学报》2016年第2期199-203,共5页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31560573);2015年度海南省普通高等学校研究生创新科研课题

摘　　要：以文心兰茜种(Oncidium Gower Ramseg)‘黄金2号’鲜切花为实验材料,对其花被不同开放阶段花被酶活性进行了研究。结果表明,花瓣与花萼的过氧化物酶活性在花发育和衰老过程中不断上升,超氧化物岐化酶活性在花盛开前期上升至峰值,之后呈现下降趋势,而花瓣与花萼的蛋白酶活性在花发育初期缓慢升高,分别在盛开前期和半开放期达到最大值,之后急剧下降。本研究揭示了3种酶活性在文心兰切花不同开放阶段的变化模式,不仅可以用来阐述文心兰切花的衰老机理,还可为延长文心兰切花寿命的基因工程育种提供依据。An attempt was made to determine the changes in enzyme activities of petals and sepals of cut-flower Oncidium orchid at different opening stages to study the physiological mechanism of senescence of orchid cut flowers. The results showed that peroxidase（ POD） activity continuously increased during the whole life span of the flower,and that superoxide dismutase（ SOD） activity rose to the peak at the pre-blooming stage and then decreased,while protease activity of the petals and sepals increased slowly,reached a maximum value at the pre-blooming and half-opened stage,then decreased sharply in the petals and sepals during the development and senescence of Oncidium orchid cut flower,respectively. This study reveals the changing modes of activities of three enzymes in the petals and sepals of cut flowers of Oncidium orchid at different opening stages. The results can be used to explain the mechanism of Oncidium flower senescence and provide the basis for varietal improvement of Oncidium orchid with longer vase life through genetic engineering in the future.

关 键 词：文心兰 过氧化物酶 超氧化物岐化酶 蛋白酶 

分 类 号：S682.31[农业科学—观赏园艺]