3个鸡种NKX2-5基因多态性与胫长的关联性分析  

Polymorphism and association analysis of NKX2-5 gene with shank length in three chickens

作　　者：杨红 张勇[2] 何琦 李俊[2] 张雄[2] 彭松[2] 

机构地区：[1]毕节市农业经营管理站,贵州毕节551700 [2]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室/贵州大学动物科学学院/贵州省动物遗传育种与繁殖重点实验室,贵州贵阳550025 [3]贵阳市花溪区农业局,贵州贵阳550025

出　　处：《广东农业科学》2016年第6期156-162,共7页Guangdong Agricultural Sciences

基　　金：贵州省农业动植物育种专项(黔农育专字[2012]10号);浙江大学基本科研业务费专项(2012XZZX003-1)

摘　　要：以贵州特有鸡种高脚鸡、矮脚鸡和百宜黑鸡为试验对象,利用PCR-SSCP和DNA测序等方法检测了NKX2-5基因T563C、C675T位点在3个试验鸡群中不同基因型与胫长的关联性。T563C与胫长关联分析表明,高脚鸡群体中TT基因型个体胫长显著高于CC型,百宜黑鸡群体中TT基因型显著高于CT型,矮脚鸡群体中不同基因型个体间胫长差异不显著;C675T突变位点在3个鸡种中只有CC和CT两种基因型,且各鸡种不同基因型个体间胫长差异不显著。Gaojiao chicken,Aijiao chicken and Baiyi black chicken in Guizhou province were used as experimental subjects in this study. SNPs of NKX2-5 genes were detected in three chicken vareties by PCR-SSCP and DNA sequencing technologies. The 2 SNPs were detected in the 3 chicken groups,T563 C,C675T sites of NKX2-5 gene were missense mutations,The relevance between T563 C site of NKX2-5 gene and chicken shank length showed that,TT genotype was significantly higher than CC genotype among Gaojiao chicken groups（P 0.05）,TT genotype was significantly higher than CT genetype in Baiyi black chicken groups（P 0.05）,uhile no significant level aruong 3 genotypes in Aijiao chicken. The C675 T site of NKX2-5 had only CC and CT genotypes,and there was no significant level in different genotypes of 3 vareties.

关 键 词：NKX2-5基因 鸡 胫长 SSCP 多态性 

分 类 号：S813.3[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]