丹参酮ⅡA对脓毒症大鼠心肌细胞高迁移率族蛋白-1、肿瘤坏死因子-α表达的影响及机制  

Influence and Mechanism of Tanshinone ⅡA on the Expression of HMGB-I and TNF-α of Cardiomyocyte in Rats with Sepsis

作　　者：汪德坤 魏汉维 彭戬 

机构地区：[1]湖北省武汉市东湖医院,湖北武汉430074

出　　处：《中国中医急症》2016年第7期1272-1273,1284,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30500657);湖北省自然科学基金(211CDB196)

摘　　要：目的观察丹参酮ⅡA对高迁移率族蛋白B1(HMGB-I)、肿瘤坏死因子-α(TNF-α)在脓毒症大鼠心肌细胞的表达的影响。方法雄性SD大鼠45只随机分为正常组(A组)、脓毒症组(B组)及丹参酮ⅡA+脓毒症组(C组),采用HE染色和ELISA法测定心肌组织中的TNF-α含量,Western blot法检测心肌组织中HMGB-1的表达。结果 HE染色显示B组大鼠心肌纤维水肿、疏松,间质充血水肿,胶原纤维增多,可见部分心内膜内皮细胞水肿脱落,而C组心肌病理改变较B组轻。ELISA法测定心肌组织TNF-α含量,Western blot法检测心肌组织中HMGB-1的表达,B组都较C组明显增加(P<0.05),心肌凋亡指数B组与C组也有明显差异(P<0.05)。且以上改变C组与A组也存在差异(P<0.05)。结论丹参酮ⅡA可以减少脓毒症大鼠心肌组织TNF-α及HMGB-I的表达,对脓毒症心肌抑制有保护作用。Objective： To observe the influence and mechanism of tanshinone Ⅱ A on the expression of HMGB-Ⅰ and TNF-α of cardiomyocyte in rats with sepsis. Methods： 45 male SD rats were randomly divided into 3 groups： the normal group（group A）,sepsis group （group B）,tanshinone Ⅱ A ＋sepsis group （group C）,using HE staining and ELISA method to determine the content of TNF-α in myocardial tissue,Western blot method to detect the expression of HMGB-1 in myocardial tissue. Results： HE staining showed that in group B,there were myocardial fibers edema,osteoporosis,interstitial hyperemia and edema,increased collagen fibers,the visible part of the endoeardial endothelial edema,and myocardial pathological changes compared with group B and group C. Group B was significantly increased compared with the group C in ELISA method to determine content of TNF-α in myocardial tissue and Western blot method to detect the expression of HMGB-1 in myocardial tissue （P〈 0.05）,and myocardial apoptosis index of group B and group C also had obvious difference（P〈0.05）. And the changes above and the differences also existed in the comparison between group A and group C （P 〈 0.05 ）. Conclusion： Tanshinone Ⅱ A can reduce the expression of TNF-α and HMGB - Ⅰ of myocardial tissue of rat in sepsis ,has a protective effect to myocardial inhibition in sepsis.

关 键 词：脓毒症 丹参酮ⅡA 高迁移率族蛋白-1 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]