侧脑室注射丹参酮对脑瘫模型大鼠神经行为学的影响  

Effect of Intracerebroventricular Injection of Tanshinone on Neurobehavioral Model of Cerebral Palsy Rats

作　　者：刘清忠[1] 王大斌[1] 韩丽梅[1] 曹觅[1] 齐旭升[1] 

机构地区：[1]湖北省十堰市太和医院湖北医药学院附属医院,湖北十堰442000

出　　处：《中国中医急症》2016年第7期1281-1284,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省科技厅项目(2013BCB002)

摘　　要：目的观察侧脑室注射丹参酮对脑瘫模型大鼠神经行为学的影响,探讨其可能机制。方法 Wistar大鼠30只,分为模型组和丹参酮组,采用手术切除大脑皮质和部分大脑组织的方法建立脑瘫模型。丹参酮尾静脉给药治疗15 d。用水迷宫仪进行定位航行和空间探索实验,并用悬吊试验和斜坡试验比较大鼠行神经行为学改变,测定治疗前后外周血丙二醛(MDA)、一氧化氮(NO)和超氧化物歧化酶(SOD)含量。结果丹参酮组大鼠水迷宫中找平台次数减少,找到平台时间和悬吊试验掉头转向上时间显著缩短,大鼠在第1象限和中环停留时间明显延长,定位航行中潜伏期和游泳距离降低,与模型组比较差异有统计学意义(P<0.05)。外周血MDA和NO明显降低,SOD明显增高,与模型组比较,差异有统计学意义(P<0.05)。结论丹参酮能促进脑瘫大鼠学习记忆能力,其作用机制有可能为丹参酮扩张大脑微动脉,增加脑组织血流量,并通过增强SOD的活性来降低MDA和NO对脑组织神经细胞的损害,达到改善大鼠缺血缺氧状态从而促进神经组织功能的恢复。Objective： To establish a rat animal model of cerebral palsy,to observe the effect of intracere- broventricular injection of Tanshinone on neurological behavior and to explore the possible mechanism of treatment of cerebral palsy. Methods： 30 Wistar rats were divided into the model group and tanshinone group, rat animal model of cerebral palsy established by surgical resection of the cerebral cortex and brain tissue. Tanshinone intravenous injection was taken for 15 days ,water maze apparatus for positioning navigation and space exploration experiments, and suspension test and ramp test rat nerve behavioral changes. Before and after treatment, content of peripheral blood MDA, NO and SOD was measured. Results： Rats of tanshinone group in Morris water maze reduce the number of finding a platform,and finding time and the time of turning up significantly shorten. Residence time in the first quadrant and the central reduced. Latency and swimming distance were decreased in positioning navigation. Compared with model group,the difference was statistically significant （P〈 0.05）. The periph- eral blood MDA and NO decreased significantly;SOD increased significantly. Compared with model group,the difference was statistically significant（P〈 0.05）. Conclusion： Tanshinone can promote learning and memory abil- ity of cerebral palsy rats,whose mechanism may be related with tanshinone＇s expanding brain arteriole,increasing cerebral blood flow,and enhancing SOD activity to reduce damage of MDA and NO to the brain nerve cells,and improve ischemia hypoxia state of rats so as to promote the recovery of nerve function.

关 键 词：丹参酮 脑瘫动物模型 大鼠 神经行为学 学习记忆能力 

分 类 号：R285.5[医药卫生—中药学]