电针足三里穴对大鼠小肠缺血再灌注损伤后肠黏膜屏障修复的影响  

Effects of Electroacupuncture at Zusanli(ST36) on Mucosal Barrier Repair in Rats with Intestinal Ischemia Reperfusion Injury

作　　者：耿艳霞[1] 陈栋[1] 鲁俊[1] 陈明祺[1] 张海东[1] 周江[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医急症》2016年第7期1300-1302,1315,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省中医院院级课题(Y14015);江苏省中医药管理局项目(JD201501)

摘　　要：目的研究电针足三里穴对大鼠小肠缺血再灌注(I/R)损伤后肠黏膜屏障的保护作用。方法将24只大鼠随机分为4组,即正常组、模型组、假电针组、电针组,每组各6只。于缺血30 min再灌注2 h时,分别取大鼠门静脉血和远端回肠标本,检测回肠组织病理学评分(Chiu′s),ELISA法检测血清中白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)浓度,Western blot法检测肠黏膜紧密连接蛋白ZO-1浓度。从回肠灌注FITC-Dextran-4溶液30 min后,测算其在血清中的浓度,观察肠黏膜通透性的改变。结果与对照组比,I/R组和假电针组大鼠病理学Chiu′s评分和血清TNF-α、IL-6、FD4水平明显升高,ZO-1表达明显减少(P<0.05);与I/R组和假电针组比,电针足三里可降低肠黏膜损伤评分,抑制血清TNF-α、IL-6和FD4水平(P<0.05),促进ZO-1修复。结论电针足三里对大鼠小肠I/R损伤肠黏膜屏障具有保护作用。Objective： To investigate the protection effect of electroacupuncture （EA） at Zusanli （ST36） on rat intestinal isehemia/repeffusion （I/R） injury. Methods： Twenty-four male SD rats were randomly divided into the control group （shame group）,I/R group （the model group）,sham EA group （I/R＋sham EA）,and EA group （I/R＋ EA） ,with 6 rats in each. Intestinal I/R rat model was based on superior mesenteric artery occlusion for 30 rain and releasing for 2h, and distal ileum tissue and portal blood samples, were taken for further detection. The pathological changes of ileum tissue were viewed and graded by Chiu＇s score. Seizure levels of IL-6 and TNF-α were detected with ELISA method. Intestinal mucosal concentrations of tight junction protein ZO-1 were investigated with western blot assay. A solution of FITC-DextranL4 solution was injected into a segment of terminal ileum for 30 min,and then the serum level of FD4 was measured to assess the intestinal permeability. Results： Compared to the control group,Chiu＇s score and serum TNF-α, IL-6,FD4 levels in I/R and sham EA group significantly increased,while ZO-1 expression significantly decreased. Compared with the I/R and shame EA group,EA at Zusanli （ST36） significantly promoted ZO-1 repair,and attenuated the pathological damage and serum levels of TNF-α, IL-6 and FD4. Conclusion： EA at Zusanli（ST36） can protect the intestinal mucosal barrier from I/R injury.

关 键 词：电针 缺血-再灌注损伤 足三里穴 肠屏障功能 

分 类 号：R245.97[医药卫生—针灸推拿学]