碳和氮源以及pH对耐有机溶剂葛根素糖基化菌株Lysinibacillus fusiformis CGMCC 4913生长及转化活性的影响  

The Impact of Carbon,Nitrogen Source as well as p H Value on the Growth and Biotransformation Activity of Lysinibacillus fusiformis CGMCC 4913,an Organic Solvent-Tolerant Glycosylation of Puerarin Producing Strain Organic

作　　者：刘贵友[1,2] 王思渊 袁生[2] 

机构地区：[1]江苏第二师范学院生命科学与化学化工学院,江苏南京210013 [2]南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与功能基因组学重点实验室,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2016年第2期61-65,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(J1103507);江苏省微生物与功能基因组学重点实验室基金(164070303402)

摘　　要：本实验从当地土壤样品中分离纯化得到一株耐有机溶剂菌株Lysinibacillus fusiformis CGMCC 4913,具有通过菌体细胞生物转化反应将葛根素转化得到葛根素-7-O-果糖苷的能力.本研究探讨碳源、氮源以及p H等培养条件对L.fusiformis CGMCC 4913菌体生长及其转化活性的影响.研究表明,LB培养基完全可以为L.fusiformis CGMCC 4913菌株生长提供碳源,蛋白胨和胰蛋白胨为氮源培养得到的菌株转化活性良好,在p H 7.0条件下LB培养基培养L.fusiformis CGMCC 4913菌株,菌株生长较好,有较高的转化活性且活性相对稳定.An organic solvent-tolerant Lysinibacillusfusiformis CGMCC 4913, isolated from the local soil sample, has the ability to convert puerarin into puerarin-7-O-fructoside by the biotransformation. This study aimed to clarify the effects of carbon sources, nitrogen sources and pH on the growth and transforming activity of L. fusiformis CGMCC 4913. The results indicated that LB medium can totally act as carbon source for L.fusiformis CGMCC 4913. And the transformation activity of the strain taking peptone and tryptone as nitrogen source performs good. LB cultured Lysinicillus fusiformis CGMCC 4913 strain grows well and exhibits relatively high and stable transformation activity.

关 键 词：葛根素 耐有机溶剂 Lysinibacillus fusiformis 培养条件 

分 类 号：Q939.99[生物学—微生物学]