金樱子黄酮与金樱子多糖对TRPV5在IgA肾病肾脏组织中表达的影响  

Effects of RLMF and RLMP on Expression of TRPV5 in Ig A Nephropathy Rat Renal Tissue

作　　者：苏上贵[1] 黄燕军[1] 黄勇奇[1] 罗广秀 张守印[1] 韦玉兰[2] 张庆梅[2] 

机构地区：[1]广西医科大学基础医学院,南宁530021 [2]广西中医药大学基础医学院,南宁530001

出　　处：《医药导报》2016年第7期702-705,共4页Herald of Medicine

基　　金：广西自然科学基金资助课题(2011GXSFA018208;2011GXSFA018272)

摘　　要：目的：观察TRPV5在IgA肾病肾脏组织中的表达,以及金樱子黄酮和金樱子多糖对TRPV5表达的影响。方法将40只SD大鼠随机分为正常对照组、模型对照组、金樱子黄酮组、金樱子多糖组。除正常对照组外,其他各组制备IgA肾病模型。金樱子黄酮组和金樱子多糖组于第9~12周分别灌服金樱子黄酮和金樱子多糖。第13周麻醉处死大鼠,提取肾脏组织RNA,荧光定量聚合酶链反应（ PCR）测定各组TRPV5 mRNA表达量。结果与正常对照组比较,模型对照组大鼠肾组织TRPV5表达减少（P〈0.01）；与模型对照组比较,金樱子黄酮组肾脏TRPV5表达差异无统计学意义（P〉0.05）,均低于正常对照组（P〈0.05）；金樱子多糖组大鼠肾脏TRPV5表达显著高于模型对照组（P〈0.01）,与正常对照组差异无统计学意义。结论金樱子多糖可通过调节TRPV5蛋白表达调节钙离子重吸收,发挥改善肾功能作用。Objective To investigate the effects of Rosa Laevigata Michx Flavoid（ RLMF） and Rosa laevigata Michx Polysaccharose （RLMP） on expression of TRPV5 in IgA Nephropathy （IgAN） rat renal tissue. Methods Forty Sprague-Dawley rats were randomly assigned to four groups.The rat model of IgA nephropathy was induced by intragastric administration of bovine serumalbumin and injections of LPS and CC14.Eight weeks later,the rats with IgAN were treated with RLMF or RLMP （4 weeks）, or normal saline.Rats was sacrificed at thirteenth weeks, and RNA was extracted from the kidney.Expression of TRPV5 in tubulointerstitial tissues were analyzed by fluorescent quantitative RT-PCR. Results After RFLP intervention,the expression levels of TRPV5 were markedly increased （P〈0.01） than model control group,while decreased （P〈0.05） than normal control group but had no significance with model control group after RFLF intervention. Conclusion TRPV5 expression is decreased in IgAN,and RLMP can adjust TRPV5 expression and improve renal function of IgAN.

关 键 词：金樱子黄酮 金樱子多糖 肾病 IgA TRPV5 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]