微波辅助提取连钱草多糖  

Microwave-assisted Extraction of Polysaccharides from Glechoma longituba

作　　者：姜宁[1] 刘晓鹏[1,2] 丁峰峻 丁振国 王秋霜[2] 梁玉堂[2] 

机构地区：[1]湖北民族学院生物科学与技术学院,恩施445000 [2]南京医药股份有限公司博士后工作站,南京210012 [3]南京同仁堂洪泽中药材科技有限公司,洪泽223200

出　　处：《医药导报》2016年第7期761-764,共4页Herald of Medicine

基　　金：国家"十二五"科技支撑计划资助项目(2011BAI04B04-05);江苏省"企业博士后集聚计划"资助项目(2011033);湖北省战略性新兴(支柱)产业人才培养项目(2013029);湖北省本科高校"专业综合改革"试点项目(2014029)

摘　　要：目的研究连钱草多糖的微波辅助提取工艺。方法通过正交实验优化水浴提取以及微波辅助提取连钱草多糖的工艺,并对两种方法进行比较。结果水浴法提取的最优工艺为液固比50∶1,时间180 min,温度90℃,p H值8.0;微波辅助提取的最优工艺为液固比30∶1、高强度的微波功率、微波时间4 min以及p H值8.0;微波辅助提取连钱草多糖的平均得率达(4.58±0.09)%,水浴法提取的平均得率为(3.09±0.12)%,微波辅助提取比水浴提取多糖提取率提高了48.2%。结论微波辅助提取连钱草多糖的工艺不仅能提高连钱草多糖的产量,且具有提取体积小、节能、省时的优点。Objective To research the microwave-assisted extraction of polysaccharides from Glechoma longituba. Methods In present study, the processes of extraction of polysaccharides from Glechoma longituba by water bath and microwave-assisted extraction were optimized using orthogonal test, and the two methods were compared. Results The optimal process of water bath extraction was as follows：liquid-solid ratio 50∶1, time 180 min, temperature 90 ℃ and pH 8. 0. The optimal parameters of microwave-assisted extraction were liquid-solid ratio 30∶1, top microwave power, time 4 min, and pH 8.0. The yield of polysaccharides from Glechoma longituba by microwave-assisted and water bath methods was （4.58±0.09）% and （3.09±0.12）%, respectively, and the yield of microwave-assisted method was elevated by 48.2% compared with that of water bath extraction. Conclusion The microwave-assisted extraction of polysaccharides from Glechoma longituba not only improves the yield, but also reduces the extract volume, saves energy and time.

关 键 词：连钱草 多糖 提取 微波辅助 

分 类 号：R286[医药卫生—中药学] TQ460.1[医药卫生—中医学]